Establishmert of rat model for orthotopic liver transplantation by double naked eye

Abstract

Objective To study the learning curve of establishing the model of othotoptic liver transplantation in the rat by a single operator with naked eyes.Methods Generalized 600 cases of surgical data in different stages,and compared the differences in the donor time,cold perfusion time,anhepatic phase,the success rate of surgery,one week survival rate,long-term survival rate and the surgical complications.Results The operation times in stage one were longer than the other three stages,the differences were statistically significant (P ＜ 0.01 ).Success rate had gradually risen from 26.7％ in stage one to almost 100.0％ in stage four,one week survival and long-term survival rate were both improved steadily.Surgical complications mainly included vascular complications in early period and biliary complications in the later period.Surgical mortality in the late three stages had a sharp decline compare to stage one.Most of surgical complications had gradually decreased,and the modeling technology was gradually stabilized.Conclusion Beginners can gradually master to establish the model of rat orthotopic liver transplantation through systemic and persistent training. Key words: Animal model; Rat orthotopic liver transplantation (ROLT) ; Learning curve