Abstract
The research of carcinogenetic mechanisms of breast cancer in different ethnic backgrounds is an interesting field, as clinical features of breast cancers vary among races. High premenopausal incidence is distinctive in East-Asian breast cancer. However, human cell lines derived from Asian primary breast tumor are rare. To provide alternative cell line models with a relevant genetic background, we aimed to establish breast cancer cell lines from Taiwanese patients of Han-Chinese ethnicity. Fresh tissue from mammary tumors were digested into organoids, plated and grown in basal serum-free medium of human mammary epithelial cells (HuMEC) with supplements. Cells were further enriched by positive selection with CD326 (epithelial cell adhesion molecule; EpCAM)-coated micro-magnetic beads. Two breast cancer cell lines derived from premenopausal women were successfully established by this method, and named Chang-Gung Breast Cancer 01 (CGBC 01) and 02 (CGBC 02). These two cell lines had a similar phenotype with weak expression of estrogen receptor (ER), progesterone receptor (PR), and without amplification of receptor tyrosine protein kinase erbB-2 (HER2/neu). Genome-wide Single Nucleotide Polymorphism (SNP) array showed multiple copy number alterations in both cell lines. Based on gene expression profiles, CGBC 01 and 02 were clustered into basal-like subtype with reference to the breast cancer cell line gene expression database. The tumorigenicity of both cell lines was extremely low in both anchorage-independence assay and transplantation into the mammary fat pads of nude mice. CGBC 01 and CGBC 02 are low tumorigenic breast cancer cell lines, established from Han-Chinese premenopausal breast cancer patients, which serve as in vitro models in studying the biological features of Asian breast cancer.
Highlights
Breast cancer is the most common malignancy affecting women globally
Two cell lines, named Chang-Gung Breast Cancer 01 (CGBC 01) and CGBC 02, were successfully established from their primary cell cultures of treatment-naïve breast tumors out of the 33 cell cultures prepared from primary tumors or metastatic cancer cells in pleural effusion (Supplementary Table 2)
CGBC (BCRC No 60610) was deposited in Bioresource Collection and Research Center (BCRC) of Taiwan and CGBC is under administrative review process for depositing
Summary
Breast cancer is the most common malignancy affecting women globally. It is the second common cause of cancerrelated death in women and is one of the most extensively studied cancer types. Establishment of two basal-like breast cancer cell lines with extremely low tumorigenicity. There are approximately 70 breast cancer cell lines established from either primary tumors or malignant pleural effusions (Supplementary Table 1). Most of these cell lines could be categorized into at least five molecular subtypes based on gene expression signatures: luminal A, luminal B, basal-like, HER2-enriched, and claudin-low [1,2,3,4]. Since breast cancer is a heterogeneous disease comprising different subtypes with different characteristics, the breast cancer cell line used should be specific to the subtype being studied [1,2,3,4,5]
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