Abstract
An efficient micropropagation system via indirect organogenesis was developed in Salacia chinensis L., an endangered anti-diabetic medicinal plant. Accurate investigation of the various plant sources (Leaf, node, shoot tip) and plant growth regulators (PGRs) impacts were accomplished in this study. Maximum rate of callus induction (93.43 ± 2.75%) was achieved from nodes inoculated on MS medium fortified with 1.0 mg/l NAA+ 2.0 mg/l BAP. Maximum shoot induction (93.33 ± 2.02%), number of shoots/explant (5.12 ± 0.09) and shoot length (3.17 ± 0.00 cm) were obtained from nodal explants inoculated on MS medium with 1.5 mg/l BAP + 1.0 mg/l NAA. IBA (2.0 mg/l) in ½ MS medium was observed to be the best rooting treatment, which promoted the highest frequency of rooting (91.66 ± 2.33%). The results suggested an efficient regeneration system for conservation and large scale production for pharmaceutical industry demands.
Highlights
Salacia chinensis L. (Celastraceae family) broadly dispersed in tropical and subtropical regions of the world, in Indian subcontinent, China and Southeast Asian countries (Muraoka et al, 2010)
Establishment of an improved, efficient and eco-friendly propagation system using different plant growth regulators (PGRs) and various explant types for conservation and large scale production of S. chinensis for industrial utilization was the main goal of the present study
MATERIALS AND METHODS Indirect organogenesis Plant material, surface sterilization and culture conditions Young and healthy shoot tips, nodal segments and leaves collected from field grown S. chinensis were washed twice in running tap water, pre-washed with concentrated dishwasher gel (3-4 drops/100 ml double-distilled water (Vim, India) for 5 min
Summary
Salacia chinensis L. (Celastraceae family) broadly dispersed in tropical and subtropical regions of the world, in Indian subcontinent, China and Southeast Asian countries (Muraoka et al, 2010). Various parts of the plant have been extensively used in various traditional medicinal systems especially as a unique Ayurvedic medicine to treat a broad range of ailments (Singh et al, 2010; Sikarwar and Patil 2012) Phytoconstituents such as mangiferin, salacinol, kotalanol, phenolic glycosides and triterpenes have been isolated from the plant which showed different therapeutic properties (Yoshikawa et al, 2003). There are only two protocols reported for the in vitro micropropagation of this endangered anti-diabetic medicinal plant (Chavan et al, 2015; Majid et al, 2016) Both the studies were limited to only direct regeneration system which is not applicable for further studies such as establishment of cell suspension culture in this plant. Establishment of an improved, efficient and eco-friendly propagation system using different plant growth regulators (PGRs) and various explant types for conservation and large scale production of S. chinensis for industrial utilization was the main goal of the present study
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