Abstract
Enzyme immobilization provides an excellent opportunity to use the enzymes for several times with greater productivity. The main aim of the present study is the establishment of a functional and sustainable bio-system engineering process based on immobilized α-amylase enzyme for the saccharification of starch with industrial focus as well as cost effective sustainable bioprocess system by using indigenous industrial waste materials as carrier agents. In this study, α-amylase was immobilized in different types of support matrices like alginate, sawdust, sugarcane bagasse and the activity of immobilized enzymes were analyzed for the breakdown of starch. The experimental results showed that the productivity of immobilized enzyme was around 2.3 times higher than the free enzyme. Conjugates of bagasse-alginate showed the highest result. A fixed batch immobilized enzyme bioreactor had been developed which could be used for the production of various valuable products in the industrial scale.
 Jahangirnagar University J. Biol. Sci. 8(1): 45-55, 2019 (June)
Highlights
Enzyme is a biocatalyst which regulates the rate of proceeding chemical reactions and it is unaltered in the process
Phenol, sodium sulfite, sulphuric acid, sodium alginate and sodium metaperiodate were purchased from Merck, India
Amount of reducing sugar produced by different support matrices without enzyme: To investigate whether the support materials have their native enzyme to breakdown the starch or not, this experiment was carried out for quantitative analysis of reducing sugar using DNS reagent treatment
Summary
Enzyme is a biocatalyst which regulates the rate of proceeding chemical reactions and it is unaltered in the process. One of the most important roles of enzymes as natural biocatalysts is their ability to increase the rate of virtually of all chemical reactions within a cell (Cooper, 2000; Robinson, 2015; Cheetham, 1987). The reactions takes a long time to be completed without the presence of catalysts. The reaction being completed within the fractions of a second in the presence of a proper enzyme (Hernandez & Fernandez-Lafuente, 2011). There are a large number of enzymes, which are being used in various industries where extremely specific catalysis is required. Some of the enzymes are cellulase, amylase, lipase, protease, papain, lignicellulase, hemicellulase, pectinase and nuclease etc
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