Abstract
The development and characterization of normal hamster tracheal epithelial (HTE) cell system and its initiated subline is described in the present study. Normal HTE cells grew in a monolayer, had a stable diploid karyotype, were anchorage dependent and non-tumorigenic. The presence of desmosomal attachments and keratin filaments confirmed the epithelial nature of these cells. An initiated subline DTC8 was isolated after treatment of HTE cells with a suboptimal dose of 9,10-dimethyl-1,2-benz[a]anthracene (DMBA). These DTC8 cells grew in a monolayer, had a higher growth rate and saturation density, were weakly anchorage independent and non-tumorigenic. Treatment of DTC8 cells with 100 ng 12-O-tetradecanoyl phorbol-13-acetate (TPA), resulted in transformation of these cells which then showed anchorage independent growth on semisolid agar and formed tumours in 85% animals. As DTC8 cells showed heterogeneity in chromosome number, they were further cloned by the limiting dilution method using gamma-irradiated hamster embryonic fibroblasts as a feeder layer. The clone H7I, isolated among these clones had all the properties of initiated cells.
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