Abstract
Human cancer stem-like cells (CSCs)/cancer-initiating cells (CICs) can be isolated as side population (SP) cells, aldehyde dehydrogenase high (ALDHhigh) cells or cell surface marker-positive cells including CD44+ cells and CD133+ cells. CSCs/CICs and non-CSCs/CICs are unstable in in vitro culture, and CSCs/CICs can differentiate into non-CSCs/CICs and some non-CSCs/CICs can dedifferentiate into CSCs/CICs. Therefore, experiments using a large amount of CSCs/CICs are technically very difficult. In this study, we isolated single cell clones from SP cells and main population (MP) cells derived from the human colon cancer cell line SW480. SP analysis revealed that SP clone cells had relatively high percentages of SP cells, whereas MP clone cells showed very few SP cells, and the phenotypes were sustainable for more than 2 months of in vitro culture. Xenograft transplantation revealed that SP clone cells have higher tumor-initiating ability than that of MP clone cells and SP clone cell showed higher chemo-resistance compared with MP clone cells. These results indicate that SP clone cells derived from SW480 cells are enriched with CSCs/CICs, whereas MP clone cells are pure non-CSCs/CICs. SP clone cells and MP clone cells are a very stable in vitro CSC/CIC-enriched and non-CSC/CIC model for further analysis.
Highlights
Cancer stem-like cells (CSCs)/cancer-initiating cells (CICs) are defined as a small subpopulation of cancer cells that are endowed with high levels of tumor-initiating ability, self-renewalPLOS ONE | DOI:10.1371/journal.pone.0158903 July 14, 2016Stable Human Cancer Stem and Non-Stem LineKobayashi foundation for cancer research
Several previous studies showed that CSCs/CICs are enriched in side population (SP) cells, not in main population (MP) cells, and we previously showed that SP cells derived from SW480 cells are enriched with CSCs/CICs [15]
SP clone cells (SP-A, SP-B, SP-C, SP-E, SP-E) showed relatively high percentages of SP cells (7.00% - 41.28%) (Fig 1B), whereas MP clone cells (MP-B, MP-C, MP-D, MP-E, MP-F, MP-G, MP-H, MP-K) showed very low percentages of SP cells (0.00% - 0.04%) (Fig 1C). These results indicate that SP clones contain higher rates of CSCs/CICs, whereas MP clones contain very few CSCs/CICs
Summary
Cancer stem-like cells (CSCs)/cancer-initiating cells (CICs) are defined as a small subpopulation of cancer cells that are endowed with high levels of tumor-initiating ability, self-renewalPLOS ONE | DOI:10.1371/journal.pone.0158903 July 14, 2016Stable Human Cancer Stem and Non-Stem LineKobayashi foundation for cancer research (to Y.H.). Cancer stem-like cells (CSCs)/cancer-initiating cells (CICs) are defined as a small subpopulation of cancer cells that are endowed with high levels of tumor-initiating ability, self-renewal. Stable Human Cancer Stem and Non-Stem Line. Kobayashi foundation for cancer research (to Y.H.).
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