Abstract
Aim: The study aimed to evaluate a modified SYBR Green qPCR method to study the inter-relations of ESR1 amplification to other parameters including ER expression status, clinicopathological features and responsiveness to tamoxifen. Materials & methods: 35 breast cancer tissues were assessed for ESR1 amplification by a modified qPCR. Results: Amplification of ESR1 was observed in 31.4% out of 35 samples. ESR1 amplification and overexpression were significantly correlated (Spearman's Rho = 0.658; p < 0.001). ESR1 amplification was also statistically associated with positive response to tamoxifen (p = 0.0005), lower tumor grade (p = 0.027) and lower tumor stage (p = 0.005). Conclusion: Findings showed a positive correlation between ER amplification and ER-α expression and highlighted the potential clinical value of using SYBR Green qPCR to quantify amplification of ESR1. ESR1 amplification could be used as an indicator of positive response to tamoxifen and might have a clinicopathological significance. However, the qPCR data should be confirmed by fluorescence in situ hybridization.
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