Abstract
For the last decade, significant attention has been paid to the occurrence, bioaccumulation and fate of drugs in effluent hospital water. Therefore, the aim of this study was to develop and validate analytical method to identify and quantify the antibiotics tetracycline HCl, (Tetra) doxycycline, (Doxy), ampicillin trihydrate (Ampi), amoxicillin trihydrate (Amoxi) and cephalexin monohydrate (Cefalex). The LCMS instrument used was equipped with with C18 column, (150 mm length x 4.6 mm inner diameter x5 um particle size). The mobile phase was acetonitrile/formic acid (1%) under gradient elution mode. The MS employs ESI unit and quadrupole mass analyzer. The analysis time was less than 15 min. The method was validated in terms of linearity, precision, accuracy, robustness, limit of detection and limit of quantitation, specificity, stability and excellent results were obtained.
Highlights
Antibiotics are natural, semisynthetic or synthetic drugs used as antibacterial, antifungal or antiparasitic
The method was validated in terms of linearity, precision, accuracy, robustness, limit of detection and limit of quantitation, specificity, stability and excellent results were obtained
During the last two decades LCMS has been extensively used in the environmental research for identification and quantification of pollutants and this due to its performance characteristics such as accuracy reproducibility, low detection limit and sensitivity
Summary
Antibiotics are natural, semisynthetic or synthetic drugs used as antibacterial, antifungal or antiparasitic. Antibiotics represent a major source of micro pollutants as they may as chemical mixtures that exhibit a wide range of mechanisms of action [4-9,1] They can undergo chemical and/or physical reactions leading different metabolites by the action of microorganisms, as well as by other physical or chemical means, resulting in mixtures with higher toxicities and risks to human health than those of the individual compounds [4,1,10-16]. In contrast to their therapeutic outcome, these antibiotics often disadvantageous for those target and non-target organisms. LC-MS method for the determination of some antibiotics in hospital waste water
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