Abstract
Lactobacillus fermentum is economically important in the production and preservation of fermented foods. A repeatable and discriminative typing method was devised to characterize L. fermentum at the molecular level. The multilocus sequence typing (MLST) scheme developed was based on analysis of the internal sequence of 11 housekeeping gene fragments (clpX, dnaA, dnaK, groEL, murC, murE, pepX, pyrG, recA, rpoB, and uvrC). MLST analysis of 203 isolates of L. fermentum from Mongolia and seven provinces/ autonomous regions in China identified 57 sequence types (ST), 27 of which were represented by only a single isolate, indicating high genetic diversity. Phylogenetic analyses based on the sequence of the 11 housekeeping gene fragments indicated that the L. fermentum isolates analyzed belonged to two major groups. A standardized index of association (I A S) indicated a weak clonal population structure in L. fermentum. Split decomposition analysis indicated that recombination played an important role in generating the genetic diversity observed in L. fermentum. The results from the minimum spanning tree strongly suggested that evolution of L. fermentum STs was not correlated with geography or food-type. The MLST scheme developed will be valuable for further studies on the evolution and population structure of L. fermentum isolates used in food products.
Highlights
Erratum The original version of this article [1] contained a mistake in the section entitled “Nucleotide sequence accession numbers”
The correct text is provided below: Nucleotide sequence accession numbers The partial sequences of the 11 multilocus sequence typing (MLST) loci used in this study have been deposited in the GenBank/EMBL databases under accession numbers KR078648KR079455,KR079658-KR080061,KP224504-KP225109, KU512980 - KU513181 and KU513182 - KU513383
R. China, Department of Food Science and Engineering, Inner Mongolia Agricultural University, Hohhot 010018, P
Summary
Erratum The original version of this article [1] contained a mistake in the section entitled “Nucleotide sequence accession numbers”. Erratum: The evolution and population structure of Lactobacillus fermentum from different naturally fermented products as determined by multilocus sequence typing (MLST)
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