Erectile dysfunction associated with aging in transgenic sickle cell disease mice (851.4)

Abstract

Priapism is defined as prolonged and persistent penile erection, unassociated with sexual interest or stimulation and increase the development of erectile dysfunction(ED), and is a complication associated with sickle cell disease (SCD). Moreover, prevalence of ED associated with priapism increases progressively with aging, but, understanding of stages involved is still poorly investigated. Thus, we evaluate the muscle reactivity of isolated corpus cavernosum (CC) of SCD old mice. The animals were divided in control (CT; 3 month), control old (CTO; 1.2 year) and SCD old (SSO; 1.2 year) groups. Concentration‐response curves to acethylcholine (ACh), sodium nitroprusside (SNP) and phenylephrine (PE) were obtained in CC mice. Nitrergic and neurogenic responses induced by electrical‐field stimulation (EFS) were also obtained in all groups. The potency (pEC50) to ACh did not change in all group, but, the maximal response (Emax) was decrease in SSO (Emax: 20 ± 9%, P<0.05) compared to CT and CTO (Emax: 95 ± 6%; 68 ± 12%, respect.). The pEC50 of SNP was decrease in SSO (5.6 ± 0,2; P<0.05) compared to CT (6.4 ± 0.2) and CTO (6.6 ± 0.1), but, the Emax did not change in all group. However, nitrergic response induced by EFS was increase in SSO mice in the higher frequencies (8‐32Hz; P<0.05) compared to CT and CTO. The pEC50 of PE did not change in SSO compared to CT and CTO, but, the Emax was increase in SSO (1.6 ± 0.1mN, P<0.05) compared to CT and CTO (Emax: 1.0 ± 0.1; 1.4 ± 0,2mN, respect.). Neurogenic contractile response was significantly decrease in the higher frequency (32 Hz) compared to CT and CTO. Thus, SSO mice exhibit an impaired ACh‐, SNP‐induced relaxant response and an increase of contractile mechanism in the CC mice featuring the ED associated with priapism in SCD.Grant Funding Source: FAPESP/CNPq