Eradication of B-lineage cells and regression of lymphoma in a patient treated with autologous T cells genetically engineered to recognize CD19

Development and Evaluation of an Optimal Human Single-Chain Variable Fragment-Derived BCMA-Targeted CAR T Cell Vector.

Novel SRRM2-Targeted CAR-T Therapy Induces Rapid Remission in a Relapsed/Refractory Multiple Myeloma Patient

T Cells Genetically Modified to Express an Anti-B-Cell Maturation Antigen Chimeric Antigen Receptor with a CD28 Costimulatory Moiety Cause Remissions of Poor-Prognosis Relapsed Multiple Myeloma

Implications of Minimal Residual Disease Negative Complete Remission (MRD-CR) and Allogeneic Stem Cell Transplant on Safety and Clinical Outcome of CD19-Targeted 19-28z CAR Modified T Cells in Adult Patients with Relapsed, Refractory B-Cell ALL

Alberta Cellular and Immunotherapy (ACIT) 001, Results of Decentralized Production of Second Generation, Anti-CD19/41BB/CD3z Chimeric Antigen Receptor (CAR) T-Cells in Relapsed/Refractory (R/R) Acute Lymphoblastic Leukemia (ALL) and Aggressive Non-Hodgkin Lymphoma (NHL)

Addition of Fludarabine to Cyclophosphamide Lymphodepletion Improves In Vivo Expansion of CD19 Chimeric Antigen Receptor-Modified T Cells and Clinical Outcome in Adults with B Cell Acute Lymphoblastic Leukemia

The BSH guideline for the management of mantle cell lymphoma (MCL)1 is under review. Pending full revision of the document, the advent of chimeric antigen receptor (CAR) T-cell therapy at third-line has prompted this addendum, the focus of which is to provide additional guidance for haematologists on the selection, investigation and surveillance of MCL patients considered potential candidates for future CAR T-cell therapy. The management of patients with MCL who progress or are intolerant to a covalent Bruton tyrosine kinase inhibitor (cBTKi) remains a significant clinical challenge.2 Tecartus, an autologous CD19-targeting CAR T-cell therapy, has been granted conditional marketing authorisation by the European Medicines Agency (EMA) for relapsed or refractory MCL after two lines of therapy, including a BTKi. The ZUMA-2 study reported impressive initial responses (overall response 93%, complete response 67%) with 37% of evaluable patients in ongoing response at a median follow-up of 35.6 months.3, 4 Overall initial responses in high-risk disease such as pleomorphic/blastoid morphology, TP53 mutations or Ki-67 proliferation index ≥50% appeared comparable but small numbers preclude valid conclusions. Significant adverse events of grade 3 or higher included cytokine release syndrome (15%), neurological events (31%) and infection (32%).3 Real-world reporting, enriched for patients with poor prognostic features, has demonstrated similar initial rates of response and toxicity.5, 6 Subsequent to approval by the National Institute for Health and Care Excellence (NICE) in February 2021, 12 centres across the UK deliver this therapy with a review of each case in England and Wales by the National CAR T Clinical Panel (NCCP) using uniform criteria (Table 1). MCL patients managed in the UK in the pre-CAR T-cell therapy era and progressing on ibrutinib represented a poor prognostic group with a post-ibrutinib median overall survival (OS) of 1.4 months for all patients and 0.4 months for those unable to receive further systemic therapy (57%).7 The latter, with a predominance of older patients, inferior Eastern Cooperative Oncology Group (ECOG) performance status (PS) and blastoid histology (32%), had achieved a median median progression-free survival (PFS) of only 3.4 months with ibrutinib, highlighting a subset with resistant and rapidly progressive disease.7 An additional pooled trial analysis and extended follow-up of 370 patients receiving ibrutinib monotherapy at relapse established that disease bulk of 5 cm or larger, raised lactate dehydrogenase (LDH), high-risk Mantle Cell Lymphoma International Prognostic Index (MIPI) score, progression of disease within 24 months of front-line therapy (POD24) and blastoid histology predict for shorter PFS and OS.8, 9 Patients from this same cohort harbouring a TP53 mutation also demonstrate poor outcomes, with a median PFS of only 4.0 months.10 Overall, the above combined population of high-risk patients represent approximately 1/3 of all MCL patients receiving ibrutinib. A significant proportion within these described cohorts will now fulfil eligibility for Tecartus. A prior history of MCL in the CNS is not an exclusion Medical co-morbidities at discretion of CAR T-cell centre Pleural effusion and ascites not an exclusion Bone marrow function: Platelets ≥75 × 109/l Neutrophils ≥1 × 109/l Lymphocytes ≥0.1 × 109/l Lower acceptable, particularly if confirmed bone marrow involvement with MCL The advent of Tecartus as third-line therapy, with the potential for durable remissions in eligible patients, warrants a review of our national approach of the investigation and surveillance of MCL at first relapse, with the goal of anticipating and capturing early refractory disease or progression on second-line ibrutinib in potential CAR T candidates with high-risk disease. Accumulating unpublished real-world UK experience with Tecartus demonstrates that pace and burden of disease are the key factors associated with failure of CAR-eligible patients reaching T-cell harvest and/or CAR T-cell infusion. A higher rate of drop-out has been observed in patients with inferior ECOG PS, blastoid histology and bulk larger than 5 cm. Such disease characteristics mirror those associated with shorter responses on ibrutinib, again reflective of a particularly poor prognostic group. With the requisite time delays built into CAR T-cell delivery (referral to a CAR centre, T-cell harvest and manufacture) CAR T-cell return can take up to eight weeks. This raises the question of the feasibility of CAR T-cell therapy in those with high-risk MCL and florid progression on second-line therapy. Theoretically, earlier referral at the first sign of ibrutinib failure may mitigate some of the risk of drop-out, improving the accessibility of CAR T-cell therapy to such patients. We recommend that all patients considered potential candidates for future CAR T-cell therapy are assessed for cBTKi failure risk and discussed with a CAR T-cell centre at first relapse according to that risk. Referral for second-line CAR T could be considered in the context of a clinical trial. Risk assessment pre-cBTKi should include up-to-date imaging and Simplified MIPI (sMIPI) status. If patients relapse with a lymphocytosis, peripheral blood should be analysed for TP53 mutations. Where practical, a repeat biopsy should be sought to assess Ki-67 proliferation index, blastoid transformation and TP53 mutation analysis if blood sampling is not possible. This information, constitutional symptoms and disease burden should be used to formulate an impression of risk of early failure of cBTKi. High-risk patients should be followed closely; at least four-weekly face-to-face appointments in the first three months. Patients with significant constitutional symptoms showing no improvement after four weeks of ibrutinib should be considered for early re-imaging. All high-risk patients should have first imaging response assessment no later than 12 weeks. Best response of stable or progressive disease should prompt an urgent referral to a CAR T-cell centre. A repeat biopsy at progression post-BTKi should not be necessary unless imaging findings are inconclusive or an alternative diagnosis is suspected. Abrupt cessation of ibrutinib at this stage should be avoided due to risk of tumour flare.11 Stabilisation of disease may be required prior to T-cell harvest and where possible, bendamustine should be omitted due to its potential impact on T-cell fitness.4 A proposed surveillance strategy is demonstrated in Figure 1. Strong predictors of long-term durable remission post CAR T-cell therapy in MCL are incompletely explored. The evidence to date is limited by small numbers and short follow-up. In a real-world dataset (n = 33), low-risk smIPI score was associated with a superior PFS.5 In high-grade B-cell non-Hodgkin lymphoma markers of disease activity (i.e., bulk, LDH), 3+ extranodal sites and inferior ECOG PS correlate with inferior survival and immediate CAR T-related toxicity post-infusion.12-15 Extrapolating this experience to MCL, adequate disease control pre-CAR T infusion may be critical to improve the drop-out rate but also to optimise the chances of durable remission and improve tolerability of Tecartus. All authors contributed to the preparation and appraisal of this manuscript. Maeve A. O'Reilly and Toby A. Eyre led on the concept and design of this document. All authors approved the final submitted version. The authors would like to thank Dr Andrew McMillan for his contribution to this work. No funding received. Maeve A. O'Reilly: advisory boards, travel and honoraria from Kite/Gilead and Novartis. Robin Sanderson: Novartis and Kite/Gilead: advisory boards, honoraria, travel. William Wilson: no conflict of interest. Sunil Iyengar: Abbvie: conference support; Beigene: advisory board; Janssen: speaker fees; Kite: advisory board, speaker fees; Takeda: advisory board, speaker fees, conference support. Jonathan Lambert: Kite: advisory boards, education, conference fees; BMS/Takeda: conference fees. Rory McCulloch: Janssen: honorarium. Toby A. Eyre: Roche: education honorarium, advisory board honorarium, travel; Gilead/Kite: Honorarium; Research support; advisory board; Janssen: Honorarium; Abbvie: honorarium; travel. AstraZeneca: honorarium, research funding, travel; Loxo Oncology: advisory board honorarium, trial steering committee; Beigene: advisory board honorarium, research funding; Incyte: advisory board honorarium; Secura Bio: advisory board honorarium. Not applicable. Not applicable.

Efficient Combinatorial Adaptor-Mediated Targeting of Acute Myeloid Leukemia with CAR T-Cells

Alberta Cellular and Immunotherapy (ACIT) 001, Updated Results of Decentralized Production of Second Generation, Anti-CD19/41BB/CD3z Chimeric Antigen Receptor (CAR) T-Cells in Relapsed/Refractory (R/R) Acute Lymphoblastic Leukemia (ALL) and Aggressive Non-Hodgkin Lymphoma (NHL)

Allogeneic CD34-Selected HSCT Following CAR T-Cells Is Associated with Low TRM and Favorable OS in Pediatric/Young Adult Patients with Relapsed/Refractory B-ALL

Radiotherapy Is an Excellent Bridging Strategy in Large B-Cell Lymphoma Patients Selected for CAR T-Cell Therapy

Antibiotic Therapy and Low Gut Microbiome Diversity Is Associated with Decreased Response and High Toxicity in BCP-ALL and DLBCL Patients after Treatment with CD19. CAR T-Cells

Significant Long-Term Benefits of CAR T-Cell Therapy Followed By a Second Allo-HSCT for Relapsed/Refractory (R/R) B-Cell Acute Lymphoblastic Leukemia (B-ALL) Patients Who Relapsed after an Initial Transplant

Chimeric antigen receptor (CAR) T-cell therapy has produced promising response rates in patients with B cell malignancies. However, previous meta-analyses have demonstrated that CAR T-cell efficacy is unsatisfactory in patients with lymphoma unlike in patient with other hematological malignancies, but these studies included insufficient numbers of studies and patients with lymphoma. Furthermore, clinicians are interested in the effects of infusion dose, CAR structure, interleukin-2 (IL-2), and conditioning therapy regimen. All clinical trials administering autologous CAR T-cell therapy in lymphoma patients were searched in medical databases. A traditional meta-analysis was performed to assess the safety and efficacy of CAR T-cells in lymphoma treatment. Subgroup analysis was performed to determine the relationships between potential factors and efficacy. The best overall response rate (ORR), 6 month ORR (6m ORR), and severe cytokine release syndrome (sCRS) rate were calculated by Stata 14.0. A total of 411 patients across all the studies were included. Our analysis showed a best ORR of 0.71, a 6m ORR of 0.63, and an overall CRS (grade ≥ 3) rate of 0.18. The subgroup analysis showed that increased response rates and reduced CRS (grade ≥ 3) rates were associated with a low dose of CAR T-cells. No IL-2 administration and the use of a fludarabine-containing lymphodepletion regimen led to improved efficacy, while anti-CD19 CAR T cells led to a more successful outcome than anti-CD20 CAR T cells. In addition, 2nd- and 3rd-generation CAR T cells exhibited increased effectiveness in clinical studies, and no significant effect diversity was found between the 2nd- and 3rd-generation CAR T cells. sCRS was associated with a high dose of infused CAR T cells when IL-2 and fludarabine were excluded from the positive factors for sCRS. CAR T cells are promising in the treatment of relapsed or refractory lymphoma. Doses lower than 10/m, no IL-2 administration, fludarabine administration, and anti-CD19 CAR T cells were related to improved efficacy and safety.

Promoter usage regulating the surface density of CAR molecules may modulate the kinetics of CAR-T cells in vivo