Equilibrium isotherm, kinetic, and thermodynamic studies of Safranin O adsorption on pomegranate (Punica granatum) peels as a low-cost adsorbent

Effect of pomegranate (Punica granatum L.) peel powder on the antioxidant and antimicrobial properties of fish gelatin films as active packaging

A total of 200 one-day-old Ross 308 male broilers were randomly assigned to 4 treatments and 5 replicates in a completely randomized design to assess growth performance, serum biochemicals, and intestinal characteristics for 42 days. Treatments comprised Control (C), probiotic, prebiotic and pomegranate peel powder (PPP). The results showed that birds fed probiotic had higher average daily feed intake (ADFI) and average daily gain (ADG) compared to the C and prebiotic groups from day 1 to 42 (p<0.05). Adding PPP improved ADFI (+2.73%) and ADG (+3.16%) compared to the C and prebiotic groups from day 1 to 42. Adding probiotic, prebiotic and PPP significantly reduced and increased feed conversion ratio (FCR) in the grower and finisher periods in comparison to C, respectively. Moreover, probiotic, prebiotic and PPP had no significant effect on FCR from day 1 to 42. Birds fed probiotic had higher villus height (VH) and lower crypt depth (CD) of the duodenum than those from other treatments (p<0.05). Birds fed probiotic and prebiotic diets had numerically lower villus surface area (VSA) in the duodenum than those in the C treatment. Probiotic supplementation enhanced the VSA in the jejunum compared to the PPP and C treatments (p<0.05). Superoxide dismutase (SOD) activity of serum increased in the probiotic and prebiotic-fed birds, but not in PPP ones (p<0.05). Probiotic, prebiotic and PPP declined serum malondialdehyde (MDA) levels in comparison to the C treatment (p<0.05). Overall, probiotic and PPP inclusion in diets improved the growth performance of broiler chicks.

The experiment aimed to evaluate the combined effects of supplementing Japanese quail diets with Moringa oleifera leaf meal (MOLM) and pomegranate peel powder (PPP) on growth, carcass quality and ileum histology. Four groups were formed: a control group, MOLM (0.2%), PPP (7.5%) and a combination of both (0.2% MOLM + 7.5% PPP). The control group exhibited the highest weight gain, followed by the MOLM group, with no significant differences in feed intake or conversion ratio. Carcass analysis showed significant differences in liver, heart and gizzard weights, with the mixed diet group having the highest values. However, wings and dressed weight percentages were unaffected by the treatments. Sensory evaluation revealed improved organoleptic properties in the mixed diet group. Histological examination demonstrated enhanced ileum morphology in the mixed diet group, with significant improvements in villi length and villus/crypt depth ratio. Overall, supplementation with 0.2% MOLM and 7.5% PPP positively impacted carcass quality, sensory attributes and bird health. These findings suggest the potential benefits of combining MOLM and PPP in quail diets to enhance growth and overall health, highlighting the importance of further research in poultry nutrition.

This study was conducted to evaluate the effect of supplementation of pomegranate peel powder on hemato-biochemical profile of lactating Mehsana goats. Twenty-four lactating Mehsana goats (7 days post- partum) were assigned randomly into four groups (6 animals in each) and stratified on the basis of their live weight and milk production in previous lactation. The experimental animals in T1 group were fed on basal diet while in T2, T3 and T4 groups were supplemented with pomegranate peel powder at the rate of 10, 20 and 40 g per day per head, respectively. The duration of this experiment was 120 days. The blood samples were collected at the end (120th day) of experimental feeding. The mean hemoglobin and packed cell values were non-significant among the experimental groups (the T1, T2, T3 and T4 groups). Likewise, there was no effect on TEC and TLC caused by the dietary addition of pomegranate peel powder in lactating Mehsana goats. The difference in serum biochemical parameters viz. glucose, total proteins, albumin, triglycerides, cholesterol, ALT, AST and urea were non-significant among T1, T2, T3 and T4 groups. The serum creatinine concentration was significantly (P<0.05) higher in groups T2 and T3 than the T1 and T4 groups. Based on the results, it may be concluded that pomegranate (Punica granatum) peel powder can be supplemented in the diet of lactating Mehsana goats up to the rate of 40 g/day/animal during the period of 120 days post-partum without any adverse effects on hemato-biochemical parameters.

Pomegranate peel powder is used as a functional ingredient in the development of nutritional bars. Pomegranate (Punica granatum) is well known fruit belongs to punicaceae family having multiple health benefits, not only limited to its edible parts but also in its non-edible parts mostly the peel. Fruit wastes are rich source of nutrients, and can be used for the development of functional food products. Pomegranate peel is considered to be beneficial due to its functional and therapeutic properties as it is a source of many biological active components like polyphenols, tannins and flavonoids. Nutrient rich and ready-made foods are the demand of everyone due to their easy availability and cost effectiveness. Among the confectionary products, bars are liked by individuals of different age groups. Hence, nutritional properties of bars can be enhanced by using pomegranate peel powder. The current study was designed to develop bars enriched with pomegranate peel powder as a basic ingredient. Pomegranate peel powder is prepared and analyzed for proximate, mineral, total phenolic content, total flavonoid content and anti-oxidant potential (DPPH). By using pomegranate peel powder, oats and jaggery, bars were prepared. In this research, five treatments T0 (0% pomegranate peel powder and 100% oats). T1 (5% pomegranate peel powder and 95% oats), T2 (10% pomegranate peel powder and 90% oats), T3 (15% pomegranate peel powder and 85% oats) and T4 (20% pomegranate peel powder and 80% oats) were used. The developed product is analyzed for proximate, mineral, total flavonoid contents, total phenolic content and anti-oxidant potential (DPPH). Proximate analysis of bars revealed that moisture, protein, fat, fiber, ash and nitrogen free extract ranges from T0 to T4 (13.38±1.21 to 11.32±1.15, 9.56±0.92 to 8.32±1.14, 9.05±1.21 to 7.93±1.08, 5.23±0.82 to 16.89±0.64, 2.05±0.87 to 2.92±1.25 and 62.51±0.85 to 52.62±0.93 respectively. Phytochemical analysis of bars enriched with pomegranate peel powder revealed that total phenolic content, total flavonoid content and antioxidant potential of bars ranges from T0 to T4 (142.74±0.65 to 211.79±0.63 mg GAE/100g, 129.16±0.64 to 192±0.53 mg QE/100g and 41.35±0.82 to 64.57±0.69%) respectively. Mineral analysis of bars enriched with pomegranate peel powder revealed that calcium, Phosphorus, Potassium, Iron, Magnesium content ranged from T0 to T4 (25.42±0.63 to 31.06±0.58, 51.00±1.01 to 45.05±1.09, 59.46±1.13 to 79.15±0.28, 1.32±1.20 to 1.95±0.83 and 54.17±0.88±0.58 to 57.36±0.68 mg/100g respectively). Sensory evaluation is done for color, aroma, taste, texture overall acceptability. T3 got maximum score. Then, the data obtained were evaluated by CRD design. On the basis of results revealed that treatment T3 with 15% pomegranate peel powder was overall highly acceptable.

This study determined the dietary effects of pomegranate peel powder meal (PPPM) supplementation on growth performance, digestibility, carcase and organ weights, serum, and some meat antioxidant enzyme indices of Cobb 500 birds. Birds (n = 432) were randomly assigned to six experimental groups of four replicates each and were fed either diets supplemented with 2, 4, 6 and 8 g/kg of PPPM (PPPM2, PPPM4, PPPM6 and PPPM8), 0% additives (Negative control; NEGCON), or with α-Tocopherol acetate at 200 g/tonne (Positive control; POSCON). The diet × week interaction effect on feed intake (FI) and feed conversion ratio (FCR) showed that the POSCON diet promoted a better FI and FCR at week 3. However, the FCR of the POSCON birds was comparable to that of the NEGCON, PPPM4 and PPPM6 birds. The average final body weight and average daily weight gain was highest in the PPPM2 and PPPM4 birds, whereas, PPPM2 birds had improved FCR and protein efficiency ratio compared with the POSCON birds. Thigh weight was highest in the PPPM4 group, whereas PPPM8 birds had the highest breast weight compared with the POSCON. Birds fed the PPPM4 diet also had improved nutrient digestibility compared with the POSCON birds. Spleen and gizzard weights were highest in PPPM4 birds compared with the NEGCON group. The concentration of serum aspartate aminotransferase (AST) was decreased in PPPM4 birds, whereas meat from PPPM8 group had the highest catalase enzyme activity. It can be concluded that birds fed 4 g/kg PPPM outperformed the birds that were fed α-tocopherol supplemented (POSCON) diets in terms of performance, digestibility, carcase and organ indices. The effect of pomegranate peel powder meal supplementation was also pronounced in decreasing the concentration of AST at 4 g/kg and increasing the activity of catalase enzyme at 8 g/kg compared with the POSCON and NEGCON diets. Highlights Dietary pomegranate peel powder can improve performance in broilers. Dietary pomegranate peel powder can enhance the enzyme activity of broiler meat.

Pomegranate (Punica granatum L.) peel is a nutritive, antioxidant rich by-product, easily available after production of pomegranate juice and ready to eat arils. The aim of present study was to utilize of pomegranate peel powder (PPP) as a good source of fiber ingredient and antioxidant bioactive compounds to produce value add bakery product based on pomegranate peel powder 2.5%, 5%, and 7.5% and investigation of weight loss activity in rats fed on high fat diet (HFD). Chemical composition, total phenols, total flavonoids and anti-oxidative activity (DPPH) of PPP and pan breads were determined. Texture profile analysis (TPA), sensory evaluation and physical properties of pan breads were investigated. Pan bread fortified with 2.5% and 5% PPP had higher score in overall acceptability and physical properties when compared to control pan bread and other various concentration (7.5%) of PPP. Thirty-six female albino adult rats with an average weight (180 ±5 g) were divided into six groups 6 rats for each. The first group (G1) fed on basal diet as a (negative control group). The other rats (30 rat) were divided as follow : (G2) fed on HFD as a positive control (PC), (G3) fed on HFD with PPP free pan bread , (G4) received HFD with 2.5% PPP fortified pan bread, (G5) received HFD with 5% PPP fortified pan bread and (G6) fed on HFD with 7.5% PPP fortified pan bread. Food intake, food efficiency, body weight, gain %, epididymal adipose tissue (g) and organs weight were recorded. Serum lipid profile and liver enzymes were analyzed. Our results showed that pomegranate peel powder 7.5% followed by 5% fortified breads reduced body weight by (47.61±1.27% and 40.65±1.11%) respectively, compared with PC group. Total lipid, triglycerides total cholesterol, LDL-C, VLDL-C and liver functions were decreased by increasing of PPP level in pan bread while HDL-C was increased .In conclusion, our results concluded that pomegranate peel powder fortified breads with high fiber and anti- oxidative activities is recommended to gain nutritional and healthy benefits to weight loss activity and the risk of obesity.

The aim of the assay was to study the effect of pomegranate peel powder (PPP) as an alternative natural additive on growth performance, carcass characteristics, and biochemical parameters of Japanese quails. The experiment involved 208 unsexed Japanese quails’ chicks initially fed a standard diet without PPP for the first week. Then, they were weighed and divided into four groups: one control group CTRL and three test groups receiving diets supplemented with 3%, 5%, and 7% PPP. Each group composed of four replicates of 13 quails. The results indicated that during the grower period, the group receiving 7% PPP showed higher feed intake (FI) (P=0.029) and feed conversion ratio (FCR) (P=0.001). However, body weight (BW) (P<0.0001), body weight gain (BWG) (P=0.001), and average daily gain (ADG) (P=0.017) were decreased. In contrast, during the finisher period, PPP supplementation did not significantly affect the final BW, BWG, or ADG (P>0.05). Notably, the groups receiving 5% and 7% PPP experienced a significant reduction in FI (P=0.001) and the 7% PPP group showed significant increases in proventriculus weight (P=0.025), relative intestine weight (P=0.017) and cecum length (P<0.0001). Furthermore, this group exhibited a noticeable decrease in albumin levels (P<0.0001) and an increase in GOT activity (P=0.002). In conclusion, PPP shows promising effects as a nutritional additive and natural growth promoter for Japanese quails. However, it is advisable to incorporate it after the grower period and to be cautious with higher doses due to potential toxicity risks.

Since adsorption is one of the best ammonium removal methods, great efforts have been made to identify new low-cost and efficient adsorbents from agricultural waste and by-products due to their abundant availability, low-cost and eco-friendly advantages, in addition to the possibility of recycling ammonium back into agricultural processes. In this study, a series of batch experiments were performed to detect new bio-adsorbents for ammonium ions removal. Among the materials tested, pomegranate peel powder showed a high affinity to adsorb ammonium ions and, furthermore, available information on ammonium adsorption by this biomaterial is still missing from the literature. First, pomegranate peel powder was characterized by the determination of different parameters such as zeta potential, iodine number, Fourier-transform infrared spectroscopy analysis, scanning electron microscopy, particle size distribution and porosity. Then, the impact of various parameters, such as pH, contact time, stirring speed, adsorbent dose and adsorbate concentration in the adsorption process, was investigated. The highest ammonium removal capacity was obtained at pH = 4 using 400 mg of pomegranate peel powder and a stirring speed of 150 rpm for an initial concentration of ammonium of 30 mg/L. The system (adsorbent, adsorbate and solution) reached equilibrium after 2 h and the data fit well with the Langmuir model with a maximum monolayer adsorption capacity of 6.18 mg/g, while kinetics were well described by the pseudo-second-order model. These results introduce pomegranate peel powder as a promising bio-adsorbent to remove and recover ammonium from aqueous solutions.

Background: Fruit – by - product includes peels, seeds, leaves, residual pulp, stems and discarded pieces from a variety of sources. Pomegranate (Punica granatum) peel is a good source of bioactive compounds, antioxidants, nutraceuticals, and functional properties and there is a healthy trend towards by-product utilization and value addition. However, its toxicity and its adverse effects were not intensively studied. Objective: This study aimed to examine the In-vivo toxicity of Pomegranate Peel Powder (PPP) extract and histopathological assessment using zebrafish (Danio rerio). Methods: Decoction (Aqueous) by Soxhlet method was used for extraction from Pomegranate Peel Powder (PPP). Dense extract was used to study toxicity level and it was assessed using Dose Dependent Toxicity Assessment (DDTA) with Zebrafish. The mature Zebrafish were divided into eight groups (A, B, C, D, E, F, G, and control) based on their average body weight, with eight fishes in each tank. Fish groups (8 fishes/concentration) were treated to different doses and concentrations of Pomegranate Peel Powder (PPP). Fish mortality was monitored and recorded after 24, 48, 72, and 96 hours. After acute toxicity analysis, H & E staining was performed to analyse the zebrafish brain. At least 3 fish from each group were taken and analysed for histopathological scoring. Result: At 24 hr and 96 hr exposure periods, the lethal dosage, to kill 50% of test fishes, was 800 mg/L. Fish treated with 200 mg/L dosage had a score grade of 1 and showed no toxic pathological changes when compared to 400 and 800 mg/L doses because they considerably had decreased pathological scores of neuronal damages, which was equivalent to the control group. Zebrafish treated with 12.5 – 200 mg/L showed no toxic effect in the brain of fish, which was comparable with the control. Conclusion: The current study showed that the No-Observed Adverse Effect Level (NOAEL) is evaluated to be 200 mg/L dosage. Thus, PPP has less toxicity, and its use is suggested with potential applications against diseases.

This study was conducted to develop chicken meat patties by incorporatingpomegranate peel and bagasse powders and their extracts. Patties were developedby incorporating pomegranate peel powder (PPP, 2 g), pomegranate aril bagassepowder (PABP, 4 g), pomegranate peel powder aqueous extract (PPAE, 6 g) andpomegranate aril bagasse powder aqueous extract (PABAE, 9 g) individually per100 g of minced meat. Both types of powders and extracts treated patties hadsignificantly higher total phenolic content than control and butylatedhydroxytoluene (BHT) treated patties. Both types of powder (PPP and PABP)treated patties had significantly higher water holding capacity, ash, crudefibre content, and hardness values, and significantly lower moisture content andlightness values in comparison to control patties. Emulsion stability andcooking yield of PABP treated patties were significantly higher than control.Addition of extracts and BHT did not influence the physico-chemical propertiesand proximate composition of chicken patties. Both types of powders and extractsprovided better protection to chicken meat patties against oxidative rancidityand microbial proliferation in comparison to control and BHT treated pattiesduring refrigerated storage. It is concluded that pomegranate fruit byproductsin the form of peel powder, aril bagasse powder and their extracts can besuccessfully utilised in development of healthier chicken meat patties and thesebyproducts can also be effectively used as a replacement of syntheticantioxidants such as BHT.

Evaluation of pomegranate (Punica granatum) peel for bioaccessibility of polyphenols and prebiotic potential using in vitro model

The highest causes of worldwide morbidity and mortality are cardiovascular diseases (CVD) that pose a major health concern affecting patients' quality of life. Pomegranate fruit contains unique compounds with various bioactivities and has been used as a powerful medicinal food in various illnesses, including CVD. Several trials confirmed the therapeutic impacts of its edible portion, but trials on pomegranate peel's impacts are fewer. Our aim was to evaluate the anti-inflammatory and antiatherogenic in vivo effects of pomegranate peel powder (PPP) in rats fed a high-fat diet (HFD). Twenty-eight albino rats were distributed among four groups: I (control), II (on HFD), III (on HFD and PPP 0.5 g/kg BW), and IV (on HFD and 1 g/kg BW). Blood samples were tested for interleukin-6 (IL-6), C-reactive protein (CRP), serum amyloid-A (SAA), total cholesterol (TC), high density lipoprotein cholesterol (HDL), and some primary biochemical parameters. HFD induced significant elevations in cholesterol and inflammatory markers as compared with controls. Nevertheless, HDL, alanine aminotransferase, creatinine, and albumin showed no change in all rats. In both PPP treatment groups, CRP and SAA levels were reduced significantly with significant decrease in TC. Samples of thoracic aorta from treatment groups showed a normal appearance and amelioration of subclinical atherosclerotic changes found in HFD group. PPP exhibited anti-inflammatory and antiatherogenic effects in comparison to HFD controls.

The peel of the pomegranate fruit is rich in polyphenols with antioxidant properties. We investigated the preventive effect of pomegranate peel (PP) powder against dextran sulfate sodium (DSS)-induced lipid peroxidation in the small intestine of rats. Rats were allocated to four groups: CONT group, fed a standard rodent diet; DSS group, fed a standard rodent diet and treated with DSS; as well as PP1%+DSS and PP5%+DSS groups, fed a standard rodent diet supplemented with either 1% or 5% of PP powder and treated with DSS. Rats of the four groups consumed their diets for 25 days. Lipid peroxidation was determined by measuring malondialdehyde (MDA) concentrations in plasma and MDA contents in the small intestine and liver. Glutathione/glutathione disulfide (GSH/GSSG) redox status and antioxidant enzyme activities were determined in the small intestine and liver. MDA content was higher (P < 0.001) in the small intestines of the DSS group compared to the CONT group. MDA content was reduced (P < 0.001) in the small intestines of the PP1%+DSS and PP5%+DSS groups compared to the DSS group. GSH contents and GSH/GSSG ratios were higher (P < 0.001) in the small intestines of the PP5%+DSS group compared to the CONT, DSS and PP1%+DSS groups. The present study demonstrates that PP powder protects the small intestine against DSS-induced lipid peroxidation by enhancing the GSH/GSSG redox potential. Powder of PP is a promising agricultural by-product containing a mixture of bioactive polyphenols that can be used for the production of functional foods aimed at the prevention of oxidative stress-induced small intestine pathogenesis. © 2021 Society of Chemical Industry.

Produced water treatment using naturally abundant pomegranate peel