Abstract

In 2007, an outbreak of epizootic hemorrhagic disease (EHD) occurred in Turkey. On the basis of clinical investigation, 41 cattle were suspected to have EHD. Reverse transcription–PCR and sequence analyses indicated that the virus belonged to EHD virus serotype 6, thus confirming EHD virus infection of cattle in Turkey.

Highlights

  • In 2007, an outbreak of epizootic hemorrhagic disease (EHD) occurred in Turkey

  • Of the selected samples submitted for bluetongue virus (BTV) and Epizootic hemorrhagic disease virus (EHDV)

  • The negative results from the blood samples may have resulted from degradation of viral RNA during transfer to the laboratory or insufficient sensitivity in the conventional RT-PCR

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Summary

Introduction

In 2007, an outbreak of epizootic hemorrhagic disease (EHD) occurred in Turkey. On the basis of clinical investigation, 41 cattle were suspected to have EHD. An initial suspicion of EHD, based on observation of clinical signs, can be confirmed by virus isolation and characterization, nucleic acid identification, or serologic testing. The cattle had stomatitis, swelling of eyelids, respiratory distress, nasal and ocular discharge, redness and scaling of muzzle and lips, lameness, and udder erythema, and some were recumbent (Table 1). Heart rates (mean 72 ± 3 beats/min) and respiratory rates (mean 24 ± 4 breaths/min) were within reference ranges of 60–80 beats/min and 10–30 breaths/min, respectively, for cattle with suspected disease.

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