Abstract
Epizootic Hemorrhagic Disease in Brocket Deer, Brazil
Highlights
Seventeen days after this first case, a 1-year-old male pygmy brocket deer (Mazama nana) from the same zoo suddenly died
To identify the suspected disease agent, we performed virus isolation and reverse transcription PCR on tissues from the pygmy brocket deer. (Virus isolation was not carried out on specimens from the gray brocket deer because brain tissue samples were inadequate and results of PCR were negative for epizootic hemorrhagic disease virus [EHDV] or bluetongue virus [BTV].) Infection with EHDV and BTV was first diagnosed by virus isolation
The phylogenetic tree shows that the partial sequence of the S10 gene segregates EHDV serogroup into 2 clusters, with LDVA (GU014478) grouping together with North American EHDV samples (Figure)
Summary
Seventeen days after this first case, a 1-year-old male pygmy brocket deer (Mazama nana) from the same zoo suddenly died. To identify the suspected disease agent (members of the species Bluetongue virus or Epizootic hemorrhagic disease virus), we performed virus isolation and reverse transcription PCR on tissues (heart, liver, lung, and bowel) from the pygmy brocket deer. (Virus isolation was not carried out on specimens from the gray brocket deer because brain tissue samples were inadequate and results of PCR were negative for epizootic hemorrhagic disease virus [EHDV] or bluetongue virus [BTV].) Infection with EHDV and BTV was first diagnosed by virus isolation.
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