Abstract
Background: Ovarian cancer is the most common fatal gynecologic malignancy in women. The BRCA2 gene has a role in regulation of cell cycle during proliferation, differentiation and DNA repair. Changes in the methylation of BRCA2 may be an effective mechanism for ovarian cancer. Objectives: The aim of the present study was to evaluate the association between ovarian cancer and methylation status of BRCA2. Materials and Methods: In this study, methylation changes of BRCA2 genes in 44 tissue samples from patients with ovarian cancer and 44 adjacent normal ovarian tissue samples were studied as the control group. After primer design and amplification of the BRCA2 gene sequence by the Polymerase Chain Reaction (PCR), gene methylation levels were evaluated using an enzymatic digestion method, Restriction Fragment Length Polymorphism (RFLP). Results: According to the study of the methylation status of subjects, status changes were observed only in three cases. The results did not show a correlation between BRCA2 gene promoter methylation in ovarian cancer patients and healthy subjects. Conclusions: According to the results obtained in this study, changes in the methylation status of BRCA2 cannot be the decisive factor to ascertain the development of ovarian cancer.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
