Abstract
MicroRNAs (miRNAs) are non-coding RNAs involved in various biological processes by regulating their target genes. Green tea polyphenol (−)-epigallocatechin-3-O-gallate (EGCG) inhibits melanoma tumor growth by activating 67-kDa laminin receptor (67LR) signaling. To examine the effect of EGCG on miRNA expression in melanoma cells, we performed miRNA microarray analysis. We showed that EGCG up-regulated miRNA-let-7b expression through 67LR in melanoma cells. The EGCG-induced up-regulation of let-7b led to down-regulation of high mobility group A2 (HMGA2), a target gene related to tumor progression. 67LR-dependent cAMP/protein kinase A (PKA)/protein phosphatase 2A (PP2A) signaling pathway activation was involved in the up-regulation of let-7b expression induced by EGCG. These findings provide a basis for understanding the mechanism of miRNA regulation by EGCG.
Highlights
Several studies have revealed that dietary polyphenols have the potential to modulate miRNA expression[24,25,26,27]
We aimed to elucidate the effect of EGCG on miRNA expression in melanoma cells and the mechanism of miRNA regulation induced by EGCG
The objective of this study was to elucidate the effect of EGCG on miRNA expression in melanoma cells and the mechanism of miRNA regulation induced by EGCG
Summary
Several studies have revealed that dietary polyphenols have the potential to modulate miRNA expression[24,25,26,27]. The modulation of miRNAs by polyphenols may be partially relevant to their physiological effects. The effects of EGCG on miRNA expression in melanoma cells are unknown. We aimed to elucidate the effect of EGCG on miRNA expression in melanoma cells and the mechanism of miRNA regulation induced by EGCG. We performed comprehensive analysis of miRNA expression profiles when melanoma cells were treated with EGCG. We assessed the involvement of 67LR signaling molecules in the miRNA regulation mechanism of EGCG
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