Abstract
A structured lipid (SL) containing n-3 fatty acids (eicosapentaenoic acid, 20:5n-3; docosahexaenoic acid, 22:6n-3) and capric acid (10:0; a medium chain fatty acid) was prepared using lipase-catalyzed acidolysis of seal blubber oil with capric acid. An immobilized lipase, Lipozyme-IM from Mucor miehei, was used as the biocatalyst. Acidolysis reactions were carried out in hexane and the products were analyzed by gas chromatography. Incorporation of capric acid was affected by mole ratio of substrates, type of organic solvent, reaction temperature, reaction time, water content and the amount of lipase. The optimum reaction mixture and conditions were oil/fatty acid mole ratio of 1:3, hexane, 45 °C, 24 h, 1% (w/w of substrates) water and 10% (w/w of substrates) Lipozyme-IM lipase. Under these conditions, a SL containing 2.3% 20:5n-3, 7.6% 22:6n-3 and 27.1% 10:0 was obtained. Solvents with log P values between 2.5 and 4.5 performed the acidolysis reaction better than those with log P values of less than 2.5. However, in the absence of any organic solvent, Lipozyme-IM afforded a satisfactory incorporation of capric acid into seal blubber oil.
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