Abstract
Microemulsions based on aliphatic hydrocarbon, surfactant and aqueous buffer have been used as reaction medium for the lipase catalyzed transesterification of a triglyceride and a fatty acid. Both AOT (sodium bis (2-ethylhexyl)sulfosuccinate) and certain alcohol ethoxylates could be used as surfactant to produce a triglyceride having a fatty acid composition similar to that of natural cocoa butter from a palm oil distillation fraction. The non-ionic surfactant gave a higher reaction rate than AOT, presumably due to a more favourable association of water in the microemulsion. Recovery of the enzyme was facile with the former surfactant. However, the ethoxylate was found to participate in an unwanted side reaction, viz, formation of esters with free fatty acids in the solution.
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