Abstract
This paper presents a label-free biosensor for the detection of single-stranded pathogen DNA through the target-enhanced gelation between gold nanowires (AuNW) and the primer DNAs branched on AuNW. The target DNA enables circularization of the linear DNA template, and the primer DNA is elongated continuously via rolling circle amplification. As a result, in the presence of the target DNA, a macroscopic hydrogel was fabricated by the entanglement of the elongated DNA with AuNWs as a scaffold fiber for effective gelation. In contrast, very small separate particles were generated in the absence of the target DNA. This label-free biosensor might be a promising tool for the detection of pathogen DNAs without any devices for further analysis. Moreover, the biosensor based on the weaving of AuNW and DNAs suggests a novel direction for the applications of AuNWs in biological engineering.
Highlights
Deoxyribonucleic acid (DNA) has significant potential as a multifunctional material, and its programmability through base-paring of DNA [1,2] has enabled the use of DNA as a building block
This paper proposes a label-free biosensor for single-stranded DNA detection induced by the interlacing of AuNW-Primer DNA conjugates (AuNW) and primer DNA branched on the AuNW
To synthesize the AuNW: DNA based biosensor, the target DNA was first designed from influenza
Summary
Deoxyribonucleic acid (DNA) has significant potential as a multifunctional material, and its programmability through base-paring of DNA [1,2] has enabled the use of DNA as a building block. The conjugation of DNA with various materials, such as gold [14,15], silver [16,17], and magnetic core [18], have endowed additional functions to the DNA nanostructures. Gold has been adopted widely for its optical and photothermal properties induced by its localized surface plasmon resonance in a variety of shapes, such as nanoparticles [19,20], nanorods [21], nanoplate films [22], and nanoclusters [23]. There are few reports on the use of gold nanowires (AuNWs) in biological engineering. AuNWs in the majority of the studies focused on gold nanoparticles assembled in a line rather than gold nanowires per se [24,25,26]
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