Abstract
The process of hydrolysis was carried out on a suspension of quinoa flour in water in a proportion of 1 in10 parts of water, previously gelatinized, to which was added an equivalent suspension of enzymes of 1,5 g/1000 g (50% of alpha amylase and 50% protease). With that, proteins were solubilized and starch was reduced to reducing su- gars, obtaining a liquid product at an average concentration of 8,5% that was later con- centrated to 45% of total solids. The hydrolysis process allows, on the basis of 1 kg of quinoa flour, toobtain80 to 85% of hydrolyzed material from20 to 15% of cellulosic residue, proteins and carbohydrates, non-digestible by the enzymes. The experiences that were carried out brought as results the following optimum values of kinetic parameters: Concentration of substrate: 10,0 %; Temperature: 60 oC; pH: 5,0 and enzyme con- centration: 1,5 g/ 1000 g. The system allows on the basis of 1 kg, the following values: percentage of hydrolyzed proteins: 74,00%; percentage of reducing sugars: 94,00%
Highlights
The process of hydrolysis was carried out on a suspension of quinoa flour in water in a proportion of 1 in10 parts of water, previously gelatinized, to which was added an equivalent suspension of enzymes of 1,5 g/1000 g (50% of alpha amylase and 50% protease)
Proteins were solubilized and starch was reduced to reducing su- gars, obtaining a liquid product at an average concentration of 8,5% that was later con- centrated to 45% of total solids
The experiences that were carried out brought as results the following optimum values of kinetic parameters: Concentration of substrate: 10,0 %; Temperature: 60 oC; pH: 5,0 and enzyme con- centration: 1,5 g/ 1000 g
Summary
The process of hydrolysis was carried out on a suspension of quinoa flour in water in a proportion of 1 in10 parts of water, previously gelatinized, to which was added an equivalent suspension of enzymes of 1,5 g/1000 g (50% of alpha amylase and 50% protease). Por ello la investigación se orientó a determinar los parámetros cinéticos de pH, temperatura y concentración de sustrato que permitan el proceso de hidrólisis de harina de “quinua” en una proporción de 1 en 10 partes de agua, previamente gelatinizada, a la cual se le adicionó una suspensión de enzimas equivalente a 1,5g/1000 g (50% de alpha amilasa y 50 % de proteasa).
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