Abstract
The research explores enhancing microalgal biomass production and its chemical composition for sustainable wastewater treatment. By using the Central Composite Design (CCD) methodology, nutrient concentrations and microbial components in the growth medium were optimized. Adjustments to light exposure, particularly with red and blue frequencies, significantly improved biomass yield, carbon sequestration, and protein content. Optimal light intensity further enhanced these parameters. A process incorporating on-demand CO2 supply and controlled pH levels showed potential for increasing biomass concentrations and nutrient utilization efficiency. Results demonstrated a 28% increase in biomass fixation and a 27% increase in biomass production compared to unoptimized conditions. The study emphasizes the effectiveness of integrated approaches in boosting biomass production and optimizing wastewater treatment, offering promising avenues for sustainable bio-based solutions
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