Abstract
Chromatin was prepared from 8-day old pea seedlings ( Pisum sativum, var Alaska) which had been treated with gibberellic acid (GA 3) for 48 h or 0 h, and its template actiivity was measured using a purified cauliflower RNA polymerase II (nucleosidetriphosphate: RNA nucleotidyltransferase, EC 2.7.7.6) added in a challenge experiment. The template activity was enhanced approx. 1.5 times by GA 3-treatment compared with control. The quantity of enzyme binding to chromatin was also increased 1.4-fold by GA 3-treatment. No significant increase in the chain elongation rate was observed and the increase in activity is ascribable to an increase of available initiation sites for transcription. An increase of reaction rate for formation of the rapid-starting complex shows that GA 3 also affects the chromatin in a way which results in binding of enzyme to chromatin more easily. These results suggest that GA 3 acts on chromatin to mediate changes in genetic transcriptional activity.
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