Abstract
The Newcastle disease vaccine strain I (ND I vaccine) was reconstituted using a 3-component combined adjuvant solution containing Astragalus polysaccharide (APS), levamisole (LMS), and selenoprotein (Se). A total of 200 Hy-Line brown chickens, 60 days of age, were randomly assigned into 10 groups. The first group was a negative control and was immunized using the phosphate buffered saline-reconstituted vaccine. The other 9 groups were immunized using the vaccine reconstituted with the combined adjuvant solution. The serum and blood samples were collected on days 7, 14, 21, 27, 35, and 42 after the vaccinations and were analyzed for IgG, IL-2, and IL-6 as well as T cell proliferation. The results showed that both the humoral and cellular immune responses were significantly enhanced compared to the negative control group. The IgG level was higher and prolonged. The IL-2 and IL-6 expressions as well as the T cell proliferation were enhanced in the experimental groups. The best combination was APS 9 mg/mL + Se 0.01 mg/mL + LMS 30 mg/mL. Our data confirmed the previous studies on immune enhancement of APS, Se, or LMS alone, but also indicated the synergy of APS + Se + LMS on the vaccine immunization.
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