Abstract
Abstract The removal of colloidal particle and lignin from a pre-hydrolysis liquor (PHL) of biomass is essential for its processing as well as for the value-added utilization of saccharides in the PHL. In this paper, pectinase was used to treat an aspen PHL obtained by pre-hydrolysis at 160 °C, and a novel process in which pectinase treatment was followed by cationic polymer flocculation to enhance colloidal particle and lignin removal was investigated. The results showed that pectinase treatment caused an approximately 50% decrease in the cationic demand of the aspen PHL. For the controlled PHL, approximately 68 mg/L poly-dimethyl diallyl ammonium chloride (pDADMAC) was needed to remove nearly all of the colloidal particles with 40.3% maximum lignin removal; for the enzyme-treated PHL, only approximately 34 mg/L pDADMAC was needed to remove nearly all of the colloidal particles with 43.3% lignin removal. In addition, the enzyme treatment decreased the loss of saccharides during subsequent pDADMAC treatment at the concentration necessary for maximum lignin removal.
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