Abstract
Cervical cancer (CVC) is one of the most common types of gynecologic malignant tumor in the world. Unfortunately, current treatments including chemo-/radiotherapy still show the limitation on CVC progress. It is well known that cancer stem cells (CSCs) plays a critical role in drug resistance and metastasis. Furthermore, dysregulation of long non-coding RNA (LncRNA) shows close association to tumorigenesis and development of multiple cancers. In this study, we investigated the cytotoxic effect of cisplatin, a clinical chemotherapeutic for cervical cancer treatment, on parental and spheroid CVC cells and surveyed the effect of LncRNA on drug-resistance. We found that spheroid CVC cells showed much more resistant to cisplatin-induced cytotoxicity compared with parental CVC cells. Furthermore, cisplatin significantly induced apoptotic cell death, while it induced cell cycle arrest in G0/G1 phase at the same dose (10 μg/ml). We also found the significant expression of EGFR in spheroid instead of parental CVC cells. Interestingly, we revealed that protruding target lncRNAs were up-regulated in cisplatin-treated spheroid CVC cells, and inhibition of these lncRNAs enhanced the cytotoxicity of cisplatin against spheroid CVC cells. These data suggest that LncRNA might act as a critical modulator on drug-resistant capability of cervical CSCs and would be a novel target for cervical cancer treatment.
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