Abstract
The culture techniques described by Mishell and Dutton (1) and Marbrook (2, 3), in which a “primary” immune response can be initiated in a suspension of mouse spleen cells to heterologous erythrocyte antigens, offer great potential for analyzing cellular and molecular events involved in humoral responses. To further exploit these techniques, manipulations of culture conditions which either stimulate or inhibit the response would offer additional advantages for biochemical studies. The present report describes the enhancement of the number of antibody-producing cells formed by mouse spleen cells in culture by the reducing agent, mercaptoethanol (MET). This enhancement was found to be a result of the influence of MET on events that occur early in the response.
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