Enhanced Wound Healing With β-Chitosan-Zinc Oxide Nanoparticles: Insights From Zebrafish Models.

Abstract

Wound healing is a complex physiological process essential for the restoration of tissue integrity and function.Novel therapeutic approaches are urgently needed to enhance wound-healing outcomes. Nanotechnology, particularly zinc oxide nanoparticles, has shown promise due to its antimicrobial, anti-inflammatory, and regenerative properties.β-chitosan, derived from squid pens, possesses superior solubility and bioactivity compared toα-chitosan, making it a valuable biomaterial for biomedical applications. Through the integration ofβ-chitosan and zinc oxide nanoparticles, this study seeks to use the complementary properties of both substances to overcome present constraints in wound care treatments. β-chitosan was extracted from squid pens and characterized for its molecular weight, degree of deacetylation, and solubility properties. Further characterization of the synthesized zinc oxide nanoparticles involved Fourier transform infrared spectroscopyto analyze chemical bonding and functional groups, ultraviolet-visible spectroscopy to determine optional properties such as band gap energy, X-ray diffraction spectroscopy to confirm the crystalline phase and calculate crystallite size, and the size was confirmed with the scanning electron microscope. Each technique provided complementary information, ensuring a comprehensive understanding of the synthesized nanoparticles' properties and their potential applications. Adult zebrafish (six to eight months old) were employed as a model organism due to their genetic similarity to humans and regenerative capabilities. Zebrafishwere wounded and divided into treatment and control groups, withβ-chitosan andβ-chitosan-derived zinc nanoparticlestreatments administrated at 50 µg/ml, while control groups received 0.05% phosphate buffer saline. The treatments, conducted in triplicate, enabled a comparative analysis of wound closure activity between β-chitosan-derived zinc nanoparticles' healing effects against standard and baseline treatments. Further, gene expression analysis on Bax, BCl-2, IL-2, IL-6, and tumor necrosis factor-alpha (TNF-a) was done using reverse transcriptase polymerase chain reaction. Characterization studies confirmed the successful synthesis ofβ-chitosan-derived zinc oxide nanoparticles and a crystalline structure corresponding to zinc oxide. Treatment withβ-chitosan-derived zinc oxide nanoparticles significantly accelerated wound closure compared to controls and other treatment groups. Microscopic analysis demonstratedenhanced epithelialization, reduced inflammatory cell infiltration, increased collagen deposition, and improved tissue organization in wounds treatedwithβ-chitosan-derived zinc oxide nanoparticles. Gene expression analysis revealed downregulation of inflammation-causing genes such as BCl-2, IL-2, IL-6, and TNF-a, hence it showed wound-healing activity. The results were statistically significant (p < 0.05). β-chitosan-derived zinc oxide nanoparticles show promising potential as a novel therapeutic strategy for enhancing wound healing. The synergistic effects ofβ-chitosan and zinc oxide nanoparticles address multiple aspects of wound healing, including antimicrobial activity, inflammation modulation, and tissue regeneration. This study highlights the advantages of nanotechnology in wound care and underscores the need for further research to optimize nanoparticle formulations for clinical applications.