Abstract
The effect of on-line photochemical reaction on UV detection in high-performance liquid chromatography (HPLC) has been studied. For on-line irradiation a photoreactor utilizing a low pressure mercury light source and a crocheted reaction capillary has been used by which the chromatographic performance of the HPLC separation is only slightly influenced. On-line irradiation of the drugs methadone, methoin, nirvanol and several barbiturate analogues results in a significant increase in their UV absorptivity, either due to the appearance of new spectral bands or due to absorption enhancement at longer UV wavelengths. By these photochemical effects, the detectability of drugs at longer UV wavelengths will be increased, in some cases more than 80-fold. Thus routine analysis of these drugs in biological samples may be simplified considerably, allowing more specific detection and less complex sample preparation.
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