Abstract
The pineapple leaves waste was exploited as a potential biomass for butanol production. The cocktail of lignocellulolytic enzymes secreted by Sphingobacterium sp. ksn- 11 employed for the saccharification of steam pretreated pineapple leaves (100 g) which produced fermentable sugars (42.89 g) and polyphenols in the hydrolysate. The polyphenols were removed selectively by using residual cornhusk bed from the hydrolysate and subjected to fermentation by Clostridium acetobutylicum NCIM 2337. The butanol tolerance and production capacity of Clostridium acetobutylicum NCIM 2337 were enhanced by supplementing histidine and proline (0.5 %) to the fermentation medium by 1.8-fold and observed increase in butanol production (4.63 g/L). In order to minimize the sub-inhibitory effect of butanol, in-situ product recovery was carried out by the addition of Dowex L-493 resin which enhanced the butanol production (6.73 g/L) by 2.6-fold and achieved maximum yield of 0.16 g/g. Thus, the one pot step optimized process for butanol production was achieved.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.