Abstract
Enhanced Optical Spectroscopy for Multiplexed DNA and Protein-Sequencing with Plasmonic Nanopores: Challenges and Prospects.
Highlights
While the reader can find exhaustive details on working principles, fabrication, and applications of plasmonic nanopores for biosensing in recent reviews,[6,7] here we focus on the applications of plasmonic nanopores as a platform for enhanced spectroscopy of single DNA and protein molecules, discussing in detail which limitations must be overcome to enable large scale multiplexing sequencing
As underlined in this paper, the key aspect in the use of plasmonic nanopores is the possibility to integrate high-sensitivity optical sensing with electrical read-out schemes
As previously discussed,[7] this approach can be used in several experiments at single-molecule levels, with the huge advantage of applying multiple methods such as fluorescence, Forster resonance energy transfer (FRET), and SERS to further enhance the amount of information that comes from a single-molecule experiment
Summary
The application of plasmonic platforms for sensing down to single-molecule sensitivity is an active research field that enables more and more advances toward different applications. In situ single cells analysis is a promising direction for the application of plasmonic nanopores because the local DNA or protein sequencing can provide valuable biological information that no other techniques can acquire. DNA data storage is an extremely interesting topic of research that is developed in parallel with single-molecule sequencing.[132−135] In this field, solid-state nanopores are used to retrieve the information stored in synthetic DNA,[132,136] and it is possible to foresee the use of optical spectroscopies to improve the degree of freedom in the binary encoding, with a primary role of plasmonic nanopores in reading the information with electro-optical read-out methods. He is the cocoordinator of the H2020FET Open DNA-FAIRYLIGHTS Project
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