Abstract
In this study, a ternary substrate mix made up of coconut pulp waste, banana peels and pineapple peels was optimally formulated to produce lipase. The optimum mix of coconut pulp waste (7.46 g), banana peels (0.01 g) and pineapple peels (7.53 g) gave a maximum lipase activity of 339.4 U/gds. The effect of inducers (castor oil, jatropha oil and olive oil) on lipase production from the substrate mix was investigated using response surface methodology (RSM) and artificial neural networks (ANN). ANN predictions were marginally superior to those of RSM. Optimized levels of castor oil (1.99%w/w), jatropha oil (0.02%w/w) and olive oil (1.99%w/w) yielded a maximum lipase activity of 488.7 U/gds which was a 30.6% increase compared to the un-induced system. Global sensitivity analysis revealed that olive oil was the most sensitive inducer. Thus, biological induction can enhance the production of lipase from the ternary substrate mix.
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