Abstract
Thermophilic bacteria pretreated sludge has drawn more interest for hydrogen production, which was more economical (needs less energy), pollution-free (adding a reagent is not needed), and efficient than physico-chemistry pretreated sludge. Comparative studies between thermophilic bacteria (Bacillus sp. AT07-1) and 65 °C heat pretreated sewage sludge have been conducted for hydrogen production. It was concluded that using thermophilic bacteria pretreated sludge for hydrogen production (batch of TB) would produce the highest hydrogen yield of 16.3 mL/g of volatile solid (VS), and it increased by 12.9, 26.4, and 52.3% compared to T + HB (65 °C heat pretreated sludge inoculated with Enterococcus sp. LG1), T (65 °C heat pretreated sludge), and TB + HB (thermophilic bacteria pretreated sludge inoculated with Enterococcus sp. LG1), respectively. The lag time of TB was only 3 h. The hydrogen concentration in the gas phase of TB + HB and T + HB was 66.2 and 67%, respectively, higher than that of TB and T. These r...
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