Enhanced fed-batch production, partial purification, characterization of jenseniin P, and discovery of a new bacteriocin-like substance produced by Propionibacterium jensenii B1264

Abstract

Propionibacterium jensenii B1264 was discovered to inhibit various Propionibacterium, Lactobacilli, and Lactococcus strains based on a deferred agar spot-on-lawn detection method in previous research, in which Lactobacillus delbrueckii ssp. lactis ATCC 4797 was the most susceptible one. The antimicrobial substance(s) was named jenseniin P and L. delbrueckii ssp. lactis ATCC 4797 was thereafter used as the indicator strain in subsequent research. In current study, production of jenseniin P was significantly enhanced via an improved fed-batch fermentation method and was partially purified using reverse phase silica gel (C18). This partially purified jenseniin P was inactivated by trypsin, pronase, and proteinase K. The molecular weight of jenseniin P was determined to be between 4.5 and 5 kDa. Jenseniin P was characterized as being pH stable with partial inactivation at 100 °C. Jenseniin P was determined to be bactericidal, causing 2.38 log reduction within 60 min at room temperature. A new bacteriocin-like inhibitory substance (BLIS) produced by P. jensenii B1264 was discovered in this study. The new BLIS inhibited the growth of Propionibacterium acnes ATCC 6919 but not the previously used indicator strain L. lactis ATCC 4797. The new discovered BLIS was recovered from the 30 % acetonitrile elution fraction of C18 resin silica column purification process. This new BLIS might present new potential application in the pharmaceutical industry for acne treatment.