Abstract
The short survival time of transplanted adipose-derived mesenchymal stem cells (ASCs) is a problem for skin wound healing. Transplantation after the formation of cellular spheroids has been investigated as a promising method for prolonging cellular survival. However, there have been technical restrictions for transplantation of spheroids in clinical practice. Here, we show an effective method for transplantation of ASC spheroids onto skin wounds in order to efficiently cure refractory ulcers. To assist anchoring of spheroids onto skin wounds, we used a 120-nm-thick free-standing film (nanosheet) that has a highly adhesive property. Bioluminescence imaging showed that ASC spheroids carried by the nanosheet survived for 14 days, which is about two-times longer than that previously reported. Wounds treated with a nanosheet carrying ASC spheroids were 4-times smaller than untreated wounds on day 14. This method for transplantation of spheroids could be applied to cell therapy for various refractory skin wounds.
Highlights
The short survival time of transplanted adipose-derived mesenchymal stem cells (ASCs) is a problem for skin wound healing
This study showed that a cell transplantation method using a nanosheet extends the lifespan of ASC spheroids for more than 10 days
ASC spheroids carried by the nanosheet promoted wound healing accompanied by enhanced secretion of vascular endothelial growth factor (VEGF) and hepatocyte growth factor (HGF), suggesting paracrine effects
Summary
The short survival time of transplanted adipose-derived mesenchymal stem cells (ASCs) is a problem for skin wound healing. Wounds treated with a nanosheet carrying ASC spheroids were 4-times smaller than untreated wounds on day 14 This method for transplantation of spheroids could be applied to cell therapy for various refractory skin wounds. There have been a few reports on the use of scaffolds for transplantation of stem cell spheroids onto skin w ounds[18,19,20], insufficient attachment between cells and tissues could limit the engraftment efficiency of ASC transplantation It has not been determined whether the use of a scaffold prolongs the activities of spheroids and enhances skin wound healing
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