Abstract
The bonding behavior between hydrophobically modified alkaline-treated gelatin (hm-AlGltn) films and porcine blood vessels was evaluated under wet conditions. Hexanoyl (Hx: C6), decanoyl (Dec: C10), and stearyl (Ste: C18) chlorides were introduced into the amino groups of AlGltn to obtain HxAlGltn, DecAlGltn, and SteAlGltn, respectively, with various modification percentages. The hm-AlGltn was fabricated into films and thermally crosslinked to obtain water-insoluble films (t-hm-AlGltn). The 42% modified t-HxAlGltn (t-42HxAlGltn) possessed higher wettability than the 38% modified t-DecAlGltn (t-38DecAlGltn) and the 44% modified t-SteAlGltn (t-44SteAlGltn) films, and the t-42HxAlGltn film showed a high bonding strength with the blood vessel compared with all the hm-AlGltn films. Histological observations indicated that t-42HxAlGltn and t-38DecAlGltn remained on the blood vessel even after the bonding strength measurements. From cell culture experiments, the t-42HxAlGltn films showed significant cell adhesion compared to other films. These findings indicate that the Hx group easily interpenetrated the surface of blood vessels and effectively enhanced the bonding strength between the films and the tissue.
Highlights
Wound closure is one of the fundamental requirements in surgical operations
Crosslinked film adhesives composed of hydrophobically modified alkaline-treated gelatin (AlGltn) with Hx (C6), Dec (C10), or Ste (C18) were fabricated and their bonding behaviors on porcine blood vessels were evaluated
The t-42HxAlGltn film with short and dense hydrophobic groups showed higher wettability, lower water content, and stronger bonding to the blood vessel compared to the other t-hm-AlGltn films
Summary
Wound closure is one of the fundamental requirements in surgical operations. A suture is usually employed to close the wounded region. Tissue adhesives have been developed and used in the clinical field for shortening the operation time and for the closure of wounds with complicated structures [1] These adhesives still possess some disadvantages in terms of bonding strength and biocompatibility [2,3,4,5,6,7,8,9,10,11]. Hydrophobically modified alkali-treated Gltns (hm-AlGltns) were prepared by the modification of the amino groups of AlGltn with fatty acid chlorides with various chain lengths, including hexanoyl (Hx: C6) chloride, decanoyl (Dec: C10) chloride, and stearyl (Ste: C18) chloride We selected those fatty acid chlorides because our previous research showed that a longer hydrophobic group such as Ste resulted in good adhesivity to tunica media [13,15]. Using the t-hm-AlGltn films, surface wettability and bonding behavior on porcine blood vessels were evaluated
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
