Engraftment of embryonic stem cell-derived hematopoietic progenitor cells is regulated by natural killer cells

Abstract

Embryonic stem (ES) cells possess the remarkable ability to form cells and tissues from all three germ layers, a characteristic known as pluripotency. In particular, the generation of ES cell‐derived hematopoietic cells could serve as an alternate source of hematopoietic stem cells for transplantation in place of bone marrow cells, which are limited by donor availability and high immunogenicity. The advantages of ES cell‐derived hematopoietic cells over bone marrow cells include a greater proliferative capacity, which alleviates the problems of donor shortage, and low level expression of MHC antigens, which suggests immune privilege. However, it is unclear whether the immune system is capable of recognizing and rejecting ES cell‐derived hematopoietic cells following transplantation. The observation that ES cell‐derivatives express low levels of MHC class I, the predominant inhibitory ligand for NK cells, led us to hypothesize that ES cell‐derived hematopoietic progenitor cells (HPC) are susceptible to NK cell‐mediated killing. To test this hypothesis, we first generated HPCs from murine ES cells ectopically expressing HOXB4, a homeobox transcription factor that confers hematopoietic self‐renewal, and confirmed that HPCs expressed low levels of MHC class I antigens. To specifically investigate the role of NK cells in regulating the in vivo engraftment of HPCs, we transplanted NK‐replete Rag2‐/‐ or NK‐deficient Rag2‐/‐γc‐/‐ mice with HPCs. We observed permanent HPC engraftment in Rag2‐/‐γc‐/‐ mice; however, HPC engraftment was significantly reduced in Rag2‐/‐ mice and was eventually eliminated over time. Bone marrow harvested from these animals showed that HPC‐derived Lin‐c‐kit+ and Lin‐Sca‐1+ progenitor cells, critical progenitor cells for long‐term hematopoietic engraftment, were deleted in Rag2‐/‐ but not in Rag2‐/‐γc‐/‐ mice.