Abstract
The aim of this study was to identify the deficiency of uridine monophosphate synthase (DUMPS) and the complex vertebral malformation (CVM) in Iranian Holstein bulls. A total of 144 blood samples were prepared of Holstein bulls in Abbas Abad Animal Breeding Center and Ferdowsi University of Mashhad's Dairy Farm in Khorasan state of Iran. Genomic PCR-RFLP protocol was performed to amplify the polymorphic region of the bovine uridine monophosphate synthase UMPS gene. Also, genomic PCR-SSCP method was performed for CVM to amplify the polymorphic region of the bovine solute carrier family 35 member 3 (SLC35A3) genes. The results of this study demonstrated that there was no carrier of DUMPS and CVM in Iranian bulls in these centers.
Highlights
Elimination of animals and species affected by inherited defects is in the interest of all concerned with animal agriculture (Robinson and Shanks, 1990)
The aim of this study was to identify the deficiency of uridine monophosphate synthase (DUMPS) and the complex vertebral malformation (CVM) in Iranian Holstein bulls
The results of this study demonstrated that there was no carrier of DUMPS and CVM in Iranian bulls in these centers
Summary
Elimination of animals and species affected by inherited defects is in the interest of all concerned with animal agriculture (Robinson and Shanks, 1990). There are several autosomal recessive genetic diseases in cattle such as deficiency of uridine monophosphate synthase (Robinson et al, 1993) and complex vertebral malformation (Steffen, 2001). The deficiency of uridine monophosphate synthase is a hereditary recessive disorder in Holstein cattle causing early embryo mortality (Kaminski et al, 2005). This enzyme has two enzymatic functions: orotic phosphoribosyl transferase (OPRTase) and orotidine monophosphate decarboxylase (OMPDCase), corres-ponding to the last two steps in pyrimidine synthesis (Patel et al, 2006). During lactation, carriers excrete an elevated level of orotic acid in milk and urine (Robinson and Shanks 1990). The embryos appear to be aborted or reabsorbed approximately 40 days after conception, leading to repeated breeding problems (Robinson et al, 1993; Lee et al, 2002)
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
