Abstract
Prostate cancer is a significant disease in men, and a large number of individuals would benefit if risk factors that increase the susceptibility to develop prostate cancer could be established, which could aid in the early detection of the disease which is crucial for successful treatment. The first objective of this study was detection of allele frequencies of 12 Y-chromosome short tandem repeat loci from Iraqian prostate cancer patients and normal control males. The second objective was to evaluate the importance of these loci to develop prostate cancer. Blood samples were collected from 70 patients unrelated males living in middle and south of Iraq. FTA® Technology was utilized to extract DNA from blood collected on FTA™ paper. Post PCR amplification was detected by using ABI Prism1 3130xl Genetic Analyzer 16-capillary array system, with POP-7™ Polymer and Data Collection Software, Genemapper version 3.5 software. A higher incidence of disease was found among males who had either allele 10 of DYS391 or allele 9 of DYS459. It is likely that Iraqi males who belong to Y-lineages with either allele 10 or allele 9 are more susceptible to develop prostate cancer, while those belonging to lineages with allele 17 of DYS456 and DYS19 are more resistant to the disease. This study shows the influence of genetic elements on prostate cancer, and it seems that DYS391 and DYS459 locus comprising with other loci have the potential to be used as a screening method for prediction of susceptibility to prostate cancer in Iraqi population. Key words: DYS459, DYS391, DYS388, DYS19, high allelic frequency, prostate cancer, STR DNA typing.
Highlights
Prostate cancer is a significant disease in men accounting for approximately 33% of all male cancers and having a 9% mortality rate for men presenting with disease (Jemal et al, 2006)
Blood samples were collected from two 70 patients unrelated males living in middle and south of Iraq and sent to the genetic laboratories comprising 35 men with prostate cancer and 35 healthy male individuals as control
All patients that participated in the study were males over 40 years old who had been referred to the hospital for treatment because of advanced level of cancer
Summary
Prostate cancer is a significant disease in men accounting for approximately 33% of all male cancers and having a 9% mortality rate for men presenting with disease (Jemal et al, 2006). A large number of individuals would benefit if risk factors that increase the susceptibility to develop prostate cancer could be M Control DNA (10 ng/μl) was added to a reaction well containing μl of PCR amplification mix. By comparison of the size of a sample’s alleles to size of alleles in allelic ladders for the same loci being tested in the sample, the STR genotyping was conducted.
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