Abstract
Successive subculturing under aerobic conditions for 14 days could be used to improve characteristics of a parent strain with a growth defect. Identification of the derived strain was based on the API 50 CHL test kit and partial sequencing of the hsp60 gene. Results show that an aerotolerant strain, Lactobacillus acidophilus DGK, was created with improved nutrition utilization. Characteristics of shorter rod-shape, higher growth rate and higher level of lactic acid concentration were observed in the domesticated strain which produced higher biomass (4.54 g dry cell weight (DCW)/L) and viable counts (9.5 x 109 CFU/mL) compared to the original strain which were 1.06 g DCW/L and 1.61 x 109 CFU/mL, respectively under aerobic conditions. Utilization of carbon and nitrogen sources was significantly improved by the derived strain except the raffinose. These results indicate that serial subculturing is a plausible method for the generation of modified strains with enhancing nutrition utilization or improving various characteristics which were beneficial in industrial processes. Key words: Biomass; serial subculture; Lactobacillus acidophilus; hsp60 gene.
Highlights
Enha anceme ent of bioma ass pro oductio on and d nutrittion utilization by b stra ain Lac ctobac cillus a acidoph hilus D
Acidophilus DGK, became aerotolerant, grew well and exhibited abundant biomass production, which would be beneficial for cell growth during fed-batch fermentation under agitation
The concentration of the cell density in the domesticated strain L. acidophilus DGK was significantly increased when cultivating while shaking, even with the different carbon sources, such as glucose, galactose, mannitol and lactose. These results indicate that the domesticated strain had been adapted to aerobic environments and exhibited higher efficiency of metabolism of the carbon sources with the exception of raffinose; biomass production was greater when the cultures were cultivated statically versus shaking
Summary
Enha anceme ent of bioma ass pro oductio on and d nutrittion utilization by b stra ain Lac ctobac cillus a acidoph hilus D. Identific cation of the e derived stra ain was base ed on the AP. Results sh how that an aerotolerantt strain, Lacttobacillus acidophilus s DGK, was created with h improved nutrition n utiliization. Utilization of ca arbon and nittrogen sourc ces was sign nificantly imp proved by the derived strain except the raffino ose. These results r indicate that seri al subculturring is a plau usible metho od for the h enhancing nutrition utiilization or im mproving va arious characteristics generation of modified strains with which were e beneficial in n industrial processes
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