Abstract
A collection of ten cultivars of tomato grown in Egypt were screened with 20 simple sequence repeat (SSR) primers in order to determine genetic identities, genetic diversity and genetic relationships among these cultivars. On an average, 38 alleles were amplified using SSR primers with scorable fragment sizes ranging from approximately 75 to 275 bp. 23 alleles were polymorphic thus revealing 60.5% of polymorphism. The genetic similarity estimated according to SSR data was scaled between 17.6 and 93.2%, suggesting the potential of SSR markers in discrimi nating among plants of close or distant genetic backgrounds. Unweighted pair group method with arithmetic mean (UPGMA) clustering grouped the cultivars into two groups where the two Egyptian cultivars Edkawy and Giza 80 were clustered in different group. In addition, clustering was found consistent with the known information regarding growth habit. The genetic distance information obtained in this study might be useful to breeder for planning crosses among these cultivars. Key words: Tomato cultivars, diversity, Simple sequence repeats (SSR), Egypt
Highlights
Tomato (Solanum lycopersicum L., formerly Lycopersicon esculentum L.) is one of the most economically important and widely grown plants in Solanaceae family
The genetic similarity estimated according to simple sequence repeat (SSR) data was scaled between 17.6 and 93.2%, suggesting the potential of SSR markers in discriminating among plants of close or distant genetic backgrounds
20 microsatellite markers were used to test the genetic diversity of ten tomato cultivars
Summary
Tomato (Solanum lycopersicum L., formerly Lycopersicon esculentum L.) is one of the most economically important and widely grown plants in Solanaceae family. In addition to its worldwide agricultural and economic importance as a crop, tomato is a preeminent model system for genetic studies in plants. Tomato is one of the most important vegetable in Egypt where it is grown all year round. Molecular genetic diversity estimates are extremely useful for intellectual property protection, in the determination of essential derivation. Measurements of genetic diversity can be used in breeding programs to increase the genetic variation in base populations by. The genetic diversity estimates based on molecular marker data may be compared to a minimum genetic distance which indicates that two cultivars are not essentially derived (Lefebvre et al, 2001)
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