Abstract
The purpose of this study is to evaluate the five candidate DNA barcodes of rbcL,matK, psbA-trnH, ITS and ITS2 in order to distinguish Taxillus chinensis from adulterants. Of the five barcodes, the amplification and sequencing efficiencies ofrbcL and trnH-psbA were 100%. Meanwhile, the efficiency of matK, ITS and ITS2 regions was very low due to failure in PCR amplification or sequencing. The psbA-trnH exhibited high inter-specific divergence and distinguished T. chinensis from other parasitic loranthus. The rbcL gene demonstrated lower inter-specific divergence and identification efficiency. In conclusion, psbA-trnH is a suitable DNA barcode in authenticating T. chinensis and its related parasitic loranthus. Key words: Taxillus chinensis, identification, DNA barcoding, psbA-trnH, Loranthaceae, parasitic loranthus.
Highlights
The purpose of this study is to evaluate the five candidate DNA barcodes of rbcL, matK, psbA-trnH, internal transcribed spacer (ITS) and internal transcribed spacer 2 (ITS2) in order to distinguish Taxillus chinensis from adulterants
The branches and leaves of Taxillus chinensis (DC.) Danser have long been used as traditional Chinese medicine for liver and kidney reinforcement, tendons and bones strengthening, relief for rheumatic conditions and abortion prevention (National Pharmacopoeia Committee, 2010)
The psbA-trnH and internal transcribed spacer (ITS) of nuclear ribosomal DNA have been proposed as supplementary barcode for further investigation (CBOL Plant Working Group, 2009)
Summary
The branches and leaves of Taxillus chinensis (DC.) Danser have long been used as traditional Chinese medicine for liver and kidney reinforcement, tendons and bones strengthening, relief for rheumatic conditions and abortion prevention (National Pharmacopoeia Committee, 2010). The psbA-trnH and internal transcribed spacer (ITS) of nuclear ribosomal DNA have been proposed as supplementary barcode for further investigation (CBOL Plant Working Group, 2009). Five proposed DNA barcodes (rbcL, matK, psbA-trnH, ITS and ITS2) has been used to identify medicinal plants (Chen et al, 2010; Yao et al, 2009; Song et al, 2009; Luo et al, 2010; Pang et al, 2010; Gao et al, 2010; Sun et al, 2010; He et al, 2010). The five proposed DNA barcodes were tested to distinguish T. chinensis from the adulterants
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