Abstract

Gladiolus is an important estimated 8th in the world cut flower trade’s cut flower grown throughout the world for its elegant attractive spikes of different hues and good keeping quality. The commercial cultivation of Gladiolus is based on natural multiplication of corms and cormels. However, multiplication rate of corms and cormels is slow and the conventional method of propagation is insufficient to meet the demand of planting material and eventually affect the final cost of corms. A number of improved conventional techniques including division of the corms, removal of leaf and flower spikes, use of standard corm size, and mechanical removal of sprouts can increase the multiplication rate of corms and cormels. These improved conventional methods of propagation are insufficient to meet the demand of planting materials. In vitro techniques are applicable for the propagation of corm producing species. These techniques are adopted at commercial level in order to fulfill supply gap of huge demand. A number of in vitro protocols have been developed for regeneration of Gladiolus plantlets using different media by using various explants sources of the plant. However, literature is rather scanty on in vitro cormel formation and acclimatization of in vitro propagules. Key words: Corms, cormels, galdiolus, propagation.

Highlights

  • African Journal of BiotechnologyNoor-Un-Nisa Memon*, Niaz Ahmad Wahocho, Tanveer Fatima Miano and Mujahid Hussain Leghari

  • Gladiolus is an important estimated 8th in the world cut flower trade grown for its elegant attractive spikes of different hues and good keeping quality (Sinha and Roy, 2002)

  • In many developing countries, the establishment cost of facilities and unit production cost of in vitro propagated plants is high, and often the return on investment is not in proportion to the potential economic advantages of the technology (Savangikar, 2004; Jo et al, 2008). This technology works in a way only when tissue culture methods are superior to conventional propagation, produce pathogen free plants in huge quantities, competition with conventional method and used for cloning

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Summary

African Journal of Biotechnology

Noor-Un-Nisa Memon*, Niaz Ahmad Wahocho, Tanveer Fatima Miano and Mujahid Hussain Leghari. A number of improved conventional techniques including division of the corms, removal of leaf and flower spikes, use of standard corm size, and mechanical removal of sprouts can increase the multiplication rate of corms and cormels. These improved conventional methods of propagation are insufficient to meet the demand of planting materials. In vitro techniques are applicable for the propagation of corm producing species. These techniques are adopted at commercial level in order to fulfill supply gap of huge demand.

INTRODUCTION
PROPAGATION OF CORMS AND CORMELS THROUGH IMPROVED CONVENTIONAL METHODS
Corm divisions and sizes
Clipping of leaves and flower spike
IN VITRO PROPAGATION OF CORMELS
Collective total weight
In vitro regeneration of cormels
GROWING MEDIUM REQUIREMENT FOR CORMEL FORMATION
Sucrose requirement for cormel formation
Use of growth retardants for cormel formation
Role of cytokinins in cormel formation
Acclimatization of in vitro propagules
Role of corm size in acclimatization
Findings
Conclusions

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