Abstract
Present investigation was planned to evaluate time period of bud breaking in Centella asiatica with different concentration of plant growth regulators, a medicinal herb distributed throughout the worldwide. For the study, concentrations were designed for response surface model describing bud breaking growth in optimum conditions. A combination of BAP (2 mg/L) + gibberellic acid (GA3, 0.5 mg/L) was achieved at a best initial bud breaking at 8thhour. Longest time period taken for bud breaking was shown in combination of BAP (0.5 mg/L) + naphthalene acetic acid (NAA, 0.5 g / L) and BAP (0.1 mg/L) + adenine sulphate (0.5 mg/L) which was recorded at 84th hour. Half strength MS media was supplemented with IBA alone (2 mg/L) and in combination with IAA (0.5 mg/L) to attain an early in vitro rooting. Their interactions observed were statistically significant (P < 0.05). Key words: Centella asiatica, bud, plant growth regulator, medicinal plant.
Highlights
Centella asiatica (L.) Urban, synonym Hydrocotyle asiatica (Family: Apiaceae) is a small perennial herb, commonly known as Mandukparni
The results of the present study on bud initiation, bud establishment and root initiation in MS medium supplemented with various combinations of growth regulators are presented in Tables 1 to 3
The earliest bud breaking in this study was achieved in BAP (2 mg/L) + gibberellic acid (GA3) (0.5 mg/L)
Summary
Centella asiatica (L.) Urban, synonym Hydrocotyle asiatica (Family: Apiaceae) is a small perennial herb, commonly known as Mandukparni. Present study was designed to understand the effect of different PGRs in alone and in combination for bud breaking, shoot formation and root initiation. Cultures were established for the bud explants on MS medium (Murashige and Skoog, 1962) containing 58 mM sucrose and gelled with 0.7% (w/v) agar.
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