Abstract
In this study, we identified novel family- and genus-specific DNA markers in Mugilidae fish. Genomic DNA was isolated from the blood of fish of 15 families and eighty (80) random primers were used for random amplified polymorphic DNA (RAPD) fingerprinting. When the primer OPAV04 was employed, a novel specific PCR product was observed in the Mugilidae family. In addition, another novel specific PCR product was also observed in the Liza genus while using primer OPAV10. Sequencing analysis revealed that the novel family- and genus-specific DNA fragments were 857 and 419 bp, respectively, and no similar sequences were found in GenBank. Two primers sets were designed based on the family- and genus-specific sequences to confirm the RAPD results and the 571 and 187 bp predicted bands were successfully amplified by PCR. Intriguingly, these two novel specific DNA markers were also effectively used for terrestrial and aquatic animal discrimination. Therefore, the novel family- and genus-specific DNA markers identified in this study can be used as an effective tool for rapid and accurate determination of the Mugilidae family and Liza genus, and even for cross-species identification. Key words: Mugilidae, family- and genus-specific sequences, DNA markers.
Highlights
The Mugilidae family fish, referred to as mullets or grey mullets, are ray-finned fish inhabiting coastal and brackish waters of all tropical and temperate regions worldwide
Mugil cephalus is a member of the Mugilidae family that migrates to the Taiwan coast and spawns in winter every
Four random primers series (OPAA, OPAV, OPAO and OPC series) were used for random amplified polymorphic DNA (RAPD)-PCR to search for a specific DNA marker among ten families of fish bought from traditional markets, supermarkets and pond cultures in Taiwan
Summary
The Mugilidae family fish, referred to as mullets or grey mullets, are ray-finned fish inhabiting coastal and brackish waters of all tropical and temperate regions worldwide. The Mugilidae family includes 17 genera and a total of 72 valid species, most classified in the genera Mugil and Liza, which have 18 and 24 species, respectively (Thomson 1997; Nelson 2006). Along the Taiwan coast, 12 species of 7 genera of Mugilidae have been recorded in “The Fish Database of Taiwan” (http://fishdb.sinica.edu.tw/). Mugil cephalus is a member of the Mugilidae family that migrates to the Taiwan coast and spawns in winter every. It is difficult to distinguish between the genera Mugil and Liza (Rossi et al, 1998a) by appearance and morphology, and the economic value of M. cehpalus is quite a bit higher than that of Liza affinis. A molecular technique must be developed to distinguish the genera Liza in the
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