Abstract
Dwarf chickling (Lathyrus cicera L) is an important forage legume crop. However, due to the presence of neurotoxin amino acid (b-N-oxalyl-L-a,b-diaminopropionic acid) in the dwarf chickling, its cultivation is limited. There is need to carry out studies for the development of varieties with reduced or none of this neurotoxin for beneficial and prolong consumption by animals. The study reports tissue culture approaches for multiplication of the plant using thidiazuron (TDZ), 6-benzylaminopurine (BAP), and α-naphthalene acetic acid (NAA) from immature zygotic embryos of the plant. The highest number (16.25) of shoots per explant was recorded on MS medium supplemented with 0.45 mg/l TDZ and 0.4 mg/l ascorbic acid. Much reduced shoot regeneration was recorded on MS medium containing different combinations BAP+NAA+ascorbic acid. The TDZ-regenerated shoots were rooted by pulse treatment with 50 mg/l IBA for 7 min. The plantlets were successfully acclimatized under the greenhouse conditions. Key words: Immature zygotic embryos, dwarf chickling, Lathyrus cicera L., axillary shoot regeneration, rooting.
Highlights
Dwarf chickling (Lathyrus cicera L.) family Fabaceae is a self-pollinated, annual, herbaceous legume plant that grows widely in Greece, Crimea, the Middle East, and Central Asia (Kislev, 1989)
We describe an efficient and reproducible protocol for rapid and large-scale propagation of L. cicera by shoot proliferation from immature zygotic embryo explants obtained from field grown plants
Axiliary shoot regeneration on MS medium containing different concentrations of BAP- naphthalene acetic acid (NAA) or TDZ was accompanied with blackening of the explant with no or diminished shoots
Summary
Dwarf chickling (Lathyrus cicera L.) family Fabaceae is a self-pollinated, annual, herbaceous legume plant that grows widely in Greece, Crimea, the Middle East, and Central Asia (Kislev, 1989) It grows in range of conditions from fertile to adverse agricultural conditions such as flooding, drought, salinity and low fertility soils, at altitude of 5 - 2000 m above sea level, in diffused bush and pine forests, rocky slopes and fields (Tubives, 2008). It flowers during April and May each year (Zohary and Hofp, 1988). Lathyrus species are widely used as animal feed in green and dry form or Abbreviations: BAP, 6-benzylaminopurine; TDZ, thidiazuron, NAA, -naphthlene acetic acid
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