Abstract
The incidence of Escherichia coli 0157: H7 was assessed in meat samples from slaughtered cattle in Ibadan metropolis by culturing on sorbitol MacConkey agar and confirmed using serological agglutination kits. The isolates were tested for susceptibility to seven commonly used antimicrobial agents. Out of the total of the 116 non-sorbitol fermenting (NSF) E. coli isolated from the samples, 71 (comprising of 18.4, 2.0, 3.2 and 4.8% from Bodija abattoir, Bashorun, Apata and Iwo Road slaughter slabs, respectively) were confirmed as E. coli O157:H7 serotype. Antibiotics susceptibility profile showed that all the isolates were resistant to one or multiple antibiotics, resulting in eight different resistance patterns. Tetracycline resistant isolates were the highest with 91.4% incidence. The results are of public health significance confirming cattle as major reservoir of EHEC and antimicrobial-resistant organisms. The high level of carcass contamination with microorganisms may be due to unhygienic slaughtering and meat processing engaged in these abattoir and slabs. Indiscriminate and misuse of antimicrobials on livestock in Nigeria could also be responsible for the multiple resistance pattern of the organism. Application of food hygiene practices such as HACCP is recommended for high quality farm to fork wholesome and safe meat for public consumption in Nigeria. Key words: EHEC, Escherichia coli, beef, food safety Ibadan, Nigeria.
Highlights
Escherichia coli is a widespread intestinal commensal organism found in human and animal resulting from fecal contamination or contamination during food animal slaughter it is often found in soil, water and foods
Shiga toxin-producing E. coli (STEC) O157 has emerged as a public health threat following its initial identification as a pathogen in a 1982 outbreak of illness associated with the consumption of undercooked ground beef (Riley et al, 1983)
Available data from United States Department of Agriculture Food Safety and Inspection Service indicated that 13 million Kg of ground beef were contaminated with E. coli O157:H7 on August 12, 1997 and 9.5 million Kg of beef trimmings and ground beef potentially contaminated with E. coli O157:H7 on July 19, 2002 (Sofos, 2008)
Summary
The incidence and antibiotics susceptibility of Escherichia coli O157:H7 from beef in Ibadan Municipal, Nigeria. The incidence of Escherichia coli 0157: H7 was assessed in meat samples from slaughtered cattle in Ibadan metropolis by culturing on sorbitol MacConkey agar and confirmed using serological agglutination kits. Out of the total of the 116 non-sorbitol fermenting (NSF) E. coli isolated from the samples, 71 (comprising of 18.4, 2.0, 3.2 and 4.8% from Bodija abattoir, Bashorun, Apata and Iwo Road slaughter slabs, respectively) were confirmed as E. coli O157:H7 serotype. Antibiotics susceptibility profile showed that all the isolates were resistant to one or multiple antibiotics, resulting in eight different resistance patterns. Indiscriminate and misuse of antimicrobials on livestock in Nigeria could be responsible for the multiple resistance pattern of the organism.
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