Abstract

Hibiscus sabdariffa calyces are mainly used for different agro-food and beverages applications. The residual biomass generated contains various useful substances that were extracted and characterized. It contained 23% (w/w) soluble pectic material, a food additive, extracted with hot acidified water (80°C, pH = 1.5) and precipitated with ethanol. The molecular weight (28.5 and 109.7 kDa), the degree of methylation (70.6 and 44.3%) and the degree of acetylation (19.0 and 4.9%) were determined for two Senegalese cultivars (koor and vimto, respectively). The effect of the extraction method on these parameters was highlighted. The residual lignocellulosic material (LCM) was chemically degraded to monosaccharides and the amount of glucose and xylose (39% of dry LCM) determined to estimate its potential as feedstock for biofuels production. However, an enzymatic degradation test revealed a recalcitrant LCM, as only 50 to 55% of its polymeric glucose content was degraded to monosaccharides without pretreatment. Xylo-oligosaccharides (XOS) are functional foods with a real market potential as prebiotics, characterized by their degree of polymerization (DP). The production of XOS synthetized by the enzymatic degradation of LCM was monitored. The results of analyses performed showed that XOS produced had mainly DP3 and DP4 values. Key words : Pectin, lignocellulosic material, enzymatic degradation, xylo-oligosaccharides.

Highlights

  • Hibiscus sabdariffa var. sabdariffa is a member of the malvaceae family

  • Combined crude extracts accounted for around 40% of calyces residues (CR) dry mass (Table 1). This was in accordance with the study of Chan and Choo (2013) who found no significant effect of water temperature (50 to 95°C) on the extraction yield of cocoa husk pectin, whereas hot acidified water helped increase pectin solubility leading to an improved extraction yield

  • Vimto and koor are the local names of two H. sabdariffa cultivars.*ratio (w/w) of enzymatic glucose or xylose to total lignocellulosic material (LCM) dry matter. **Ratio (w/w) of enzymatic glucose or xylose to total glucose or xylose content

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Summary

Introduction

Sabdariffa is a member of the malvaceae family. This plant is an erect, annual shrub characterized by large and succulent sepals rich in anthocyanins In Senegal it was traditionally used to manufacture drinks and only a small proportion of calyces were processed locally. Newly developed industrial products (spray dried instant drinks, energy drinks, bottled drinks etc.) made from dry calyces are manufactured by local small and medium facilities. Due to environmental and economic considerations agro-industrial byproducts are processed to recover and/or produce valuable products. To date H. sabdariffa’s residual biomass has not been considered with the objective of increasing its value

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