Abstract
In this paper, ratio derivative UV spectroscopic method was developed and validated for the determination of thiamethoxam residue in tea. To estimate thiamethoxam, the amplitude of the first derivative of the corresponding ratio spectra at 258nm was used. Correlation coefficient of the calibration model was considered to be 0.9977. Besides the method was validated according to ICH guidelines by assessing the linearity, accuracy, limit of quantification, limit of detection. The results demonstrate that ratio derivative spectrophotometry is suitably applied for the detection of thiamethoxam residue in tea.
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