Abstract
The objective of this study is to investigate the optimal parameter levels for removal of nitrates from wastewater using Pseudomonas stutzeri in a fluidized bed bioreactor by Taguchi method and validating the predicted values. In this study the influence of airflow rate, temperature, carbon source, PP beads (Poly Propylene) and pH at different levels was investigated was made using Taguchi's L16(4) 5 orthogonal array technique. The nitrate removal efficiency after optimization is more than 96% with airflow rate at level 2 (A2), temperature at level 3(B3), carbon source at level 4(C4), PP beads at level 2(D2) and pH at level 2(E2). The analysis of variance (ANOVA) test is used to determine the percent contribution of each parameter and found that airflow rate contribution is the highest and temperature provides the least contribution. Keywords - Wastewater, Biological denitrification, Fluidized bed bioreactor, Pseudomonas stutzeri, Taguchi method,
Highlights
Nitrate is a major pollutant present in effluent wastewater from nitrogenous fertilizer, metal ores, explosives, paper mills, pulp mills, explosive industries and from municipal waste
Several studies has been carried on denitrification [6,7,8,9,10,11,12,13,14] but work reported on biological denitrification of waste water using a fluidized bed bioreactor is very little using Pseudomonas stutzeri
Taguchi method is an effective tool for optimizing the parameters for the denitrification of wastewater and the procedure reduces the practical performances for experimentation and the approach is easy to implement
Summary
Nitrate is a major pollutant present in effluent wastewater from nitrogenous fertilizer, metal ores, explosives, paper mills, pulp mills, explosive industries and from municipal waste. Each of them has its own advantages and disadvantages Among these conventional means for NO3-N removal from drinking water include ion exchange and variety of membrane technologies, such as reverse osmosis. These processes have been proven effective in NO3-N removal [5]. 2001 [18], reported on new methods like ion exchange, reverse osmosis and electro-dialysis for nitrate removal from water and that the utility of these processes has been limited due to their expensive operation and subsequent disposal problem of the generated nitrate waste brine. Yuguang Li et al, 2007 [20] reported optimization process parameter of petroleum bio desulfurization by Taguchi methodology
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