Abstract
Thirty tissue samples were examined histologically and classified as being inflamed (apical granulomas) or noninflamed (scars or noninflamed cysts). The samples were then homogenized in pyrogen-free water and treated to remove interfering substances. The presence of endotoxin was then determined by means of the Limulus assay; 75 percent of the inflamed tissues were positive for endotoxin, while only 20 percent of the noninflamed tissues contained endotoxin. The presence of endotoxin was thus highly correlated (p = 0.015) with the presence of inflammation in these tissues.
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